Study on the HN Gene Cloning of NDV Isolates in South China and their Phylogenesis

HE Dong—sheng; QIN Zhi—Ieng; LIU Fu an

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April 2002

HE Dong—sheng, QIN Zhi—Ieng and LIU Fu an. Study on the HN Gene Cloning of NDV Isolates in South China and their Phylogenesis[J]. Virologica Sinica, 2002, 17(2): 163-165.

Citation: HE Dong—sheng, QIN Zhi—Ieng, LIU Fu an. Study on the HN Gene Cloning of NDV Isolates in South China and their Phylogenesis .VIROLOGICA SINICA, 2002, 17(2) : 163-165.

华南地区新城疫病毒HN基因的克隆及其系统发育分析

1.
华南农业大学动物医学系，广州519642

摘要

用RT-PCR一步法扩增了华南地区NDV野毒HN基因．并对其中的3株的HN基因897bp片段进行了克隆、测序及同源性分析。本试验还对该毒株的系统发育情况进行了分析：中国华南地区NDV各毒株与欧美国家I 至V基因型明显分离．遗传关系较远台湾省的NDV各毒株归属为一型．并且与华南株的遗传距离较近。NDV 华南FS1株不能归娄到任何一十基因型中。广东地区从鹅群中分离到的副粘病毒GPMV株与我们从鸡体中获得的NDV FS2株有很近的亲缘关系。
- 新城疫病毒
- , HN基因
- , 系统发育

Study on the HN Gene Cloning of NDV Isolates in South China and their Phylogenesis

1.
Vet Dept，South China Agr~’cuftural University，Guangzhou，510642．China

Abstract

Three HN genes of NDV isolates from South China were am plified by one-step RT-PCR ． They where subsequently cloned and sequenced． The Phylogenesis of these NDV isolates was also studied．The gene types of So uth China NDV strains were found to be separated from those of Eum — Ameriean NDV strains exhibiting farther genetic distance NDV isolates from Taiwan Province be， longed to the same gene type as those from Mainland China．However，one of the Chinese NDV strain， FS1．belonged to none of these gene types．Significantly，a paramyxovirus strain．named GPM V that was isolated ftom Guangdong Province，had a rather close genetic relationship with FS2 stain that was isolated from chicken in Guangdong
- Newcastle Disease Virus
- , HN gene
- , PhylogenisiS

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the HN Gene Cloning of NDV Isolates in South China and their Phylogenesis

1. Vet Dept，South China Agr~’cuftural University，Guangzhou，510642．China

Abstract: Three HN genes of NDV isolates from South China were am plified by one-step RT-PCR ． They where subsequently cloned and sequenced． The Phylogenesis of these NDV isolates was also studied．The gene types of So uth China NDV strains were found to be separated from those of Eum — Ameriean NDV strains exhibiting farther genetic distance NDV isolates from Taiwan Province be， longed to the same gene type as those from Mainland China．However，one of the Chinese NDV strain， FS1．belonged to none of these gene types．Significantly，a paramyxovirus strain．named GPM V that was isolated ftom Guangdong Province，had a rather close genetic relationship with FS2 stain that was isolated from chicken in Guangdong