Analysis of the Structure and Prokaryotic Expressing of the Glycoprotein G Gene of Pseudorabies Virus

MIN Ping; ZHANG Chu—yu; PAN Zi—shu

PDF
Cite
Share

facebook

twitter

google

linkedin

April 2002

MIN Ping, ZHANG Chu—yu and PAN Zi—shu. Analysis of the Structure and Prokaryotic Expressing of the Glycoprotein G Gene of Pseudorabies Virus[J]. Virologica Sinica, 2002, 17(2): 166-171.

Citation: MIN Ping, ZHANG Chu—yu, PAN Zi—shu. Analysis of the Structure and Prokaryotic Expressing of the Glycoprotein G Gene of Pseudorabies Virus .VIROLOGICA SINICA, 2002, 17(2) : 166-171.

伪狂犬病病毒糖蛋白G基因的结构分析及其原核表达

1.
武汉大学病毒学研究所，武汉430072

摘要

利用PCR技术扩增曲狂犬病毒湖北抹(PRV liB)糖蛋白G(gG)基因．进行了序列测定和分析结果显示扩增和测序片段长1804bp．G+C含量68 78％。gG 基因ORF长1500bp，编码500个氪基酸组成的多酞与PRV Rice株gG 基因比较．两者核苷酸及推导的氪基酸序列同源性分别为98％、84 1％。320～380位之问的氮基酸序列存在较大差异。根据序列分析结果．选取gG基因长短不同的两个片段分别克隆到原棱表达载津口ET28a(+)进行表达。经SDS PAGE和Dot—ELISA分析证实表达出分子量大小分别约为551．1)和63kD的特异性gG 多酞，这为深入阐明PRV gG基因结构与功能及研制gG-ELISA诊断试剂盒奠定丁基础。
- 伪狂犬病病毒
- , 糖蛋白G
- , 桩苷酸序列
- , 氮基酸序列
- , 基因表达

Analysis of the Structure and Prokaryotic Expressing of the Glycoprotein G Gene of Pseudorabies Virus

1.
Virology Research Institule of Wuhan University，Wuhan 430072，China

Abstract

The glycoprotein G (gG)gene of pseudorahies virus Hubei strain(PRV HB)was amplified by PCR，sequenced and analyzed．The results revealed that the fragment cloned and sequenced was 1804 base pairs(bp)with a G+C content of 68．78％ and an ORF containing 1500bpto encode a pro— rein of 500 amino acids Comparison of the gG gene of PRV HB with PRV Rice strain showed the se— quence homologies of the nucteotide and deduced amino acid welne 98％ and 84．1％． respectively． Most of thes,e diferences were located within amino acid residues 320 to 380 According to the nu— cleotide sequence analysis，two different length segments of gG gene were cloned into the prokaryotic vector pET28a(+)and expre~ed．The specific polypeptides of gG．whose molecular weights were about 55kD．63kD respectlve]y，x,ere identified by SDS—PAGE and Dot—El ISA．This results might be contribute to the study of the structure and function of G gene and gG—EI ISA diagnosis kit of PRV．
- Pseudorabies virus
- , Glycoprotein G
- , Nucleotide sequence
- , Amino acid sequence
- , Gene expressing

References
Proportional views

PDF

Article Metrics

Article views(3739) PDF downloads(869) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of the Structure and Prokaryotic Expressing of the Glycoprotein G Gene of Pseudorabies Virus

1. Virology Research Institule of Wuhan University，Wuhan 430072，China

Abstract: The glycoprotein G (gG)gene of pseudorahies virus Hubei strain(PRV HB)was amplified by PCR，sequenced and analyzed．The results revealed that the fragment cloned and sequenced was 1804 base pairs(bp)with a G+C content of 68．78％ and an ORF containing 1500bpto encode a pro— rein of 500 amino acids Comparison of the gG gene of PRV HB with PRV Rice strain showed the se— quence homologies of the nucteotide and deduced amino acid welne 98％ and 84．1％． respectively． Most of thes,e diferences were located within amino acid residues 320 to 380 According to the nu— cleotide sequence analysis，two different length segments of gG gene were cloned into the prokaryotic vector pET28a(+)and expre~ed．The specific polypeptides of gG．whose molecular weights were about 55kD．63kD respectlve]y，x,ere identified by SDS—PAGE and Dot—El ISA．This results might be contribute to the study of the structure and function of G gene and gG—EI ISA diagnosis kit of PRV．