Infection Characterizations of four Grass Carp Reovirus(GCRV)Strain

FANG Qin; XIAO Tiao; IA I; ZOU Gui; ZHANG Huai; WANG Ya

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April 2002

FANG Qin, XIAO Tiao, IA I, ZOU Gui, ZHANG Huai and WANG Ya. Infection Characterizations of four Grass Carp Reovirus(GCRV)Strain[J]. Virologica Sinica, 2002, 17(2): 182-184.

Citation: FANG Qin, XIAO Tiao, IA I, ZOU Gui, ZHANG Huai, WANG Ya. Infection Characterizations of four Grass Carp Reovirus(GCRV)Strain .VIROLOGICA SINICA, 2002, 17(2) : 182-184.

四株草鱼呼肠孤病毒毒株的细胞感染特性比较研究

1.
1 中国科学院水生生物研究所．武汉430072
2.
中国科学院武汉病毒研究所，武汉430071
3.
南农业大学水产学院．长沙410128

摘要

摘要：本文首次对低温保存的三株草鱼呼肠孤病毒GCRvm、GCRvm、C-CRV 与新分离的GCRv t毒株进行了细咆培养与病毒感染特性等比较研究。结果表明，GCRV州、GCRV日7 GCRVs76在一30’(2保存l0年后仍然具有一定的感染性，其滴度均在10 TCID50／mL以上．略低于从病鱼组织分离的GCRV991毒株的滴价。经传代培养后，四株GCRV的毒力逐渐升高．并趋于稳定；当感染复数(MOI)为0 05PFu／cell时，测定四株GCRV的滴度均高于l0 TCID~／mL 但略有差异。GCRVs73的滴度品高，可达到6．4×10“1℃I n／mL。连续传代的GCRV毒株在不阿温度(28"(2、31’(2、34"12；、3TC、4112)务件下均可感染CIK鲴咆；在Z8"C时，感染效价最高，随着温度的升高．其感染效价逐渐降低。
- 草鱼呼肠孤病毒
- , 毒株
- , 感染特性

Infection Characterizations of four Grass Carp Reovirus(GCRV)Strain

1.
LI State Key Laboratory of Fresh~,ater Ecology and Biotechnology， Institute of Hydrobiotogy Chinese Academy of SciencesWuhan 430072，China
2.
Wuhan ln~t ztute of Virology，Ch inese Academy of S~a'ence$，Wuhan 430071，China
3.
Hunan Agricultural Universlty Changsha 410128．China

Abstract

Abstract：Some characterizations of four Grass Carp Reovirus(GCRV)strains were comparatively studied at the first time．It showed that the stored GCRV873，GCRVs75 and GCRv876 at minus 3012 for ten years still remained infectious to CIK cells，and the 3 virus titers could reach above 10 TCIDs0／ mI ，which was a little lower than the newly isolated GCRV99l strain s．After inoculation 3 4 genera— tions，their titers rised gradually，and the titer of GCRV873 could be up to the highest，which was al— most 6 4x10“TCIDso／mI with 0．05PFu／cellmultiplicity ofinfection(MOI)at 2812 ．Theinfec— tive ability of the four strains at different temperatures(28℃ ，3l℃ 。34℃ ，37℃ and 41℃ )was also detected，which revealed that CPE appeared in CIK cells under above conditions，but titers depressed as the temperature rised In addition，4 GCRV—ds RNAs were also compared in a same electronic con— dition。
- GrasS Carp Reovirus(GCRV)
- , Strains
- , Infection Characterization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Infection Characterizations of four Grass Carp Reovirus(GCRV)Strain

1. LI State Key Laboratory of Fresh~,ater Ecology and Biotechnology， Institute of Hydrobiotogy Chinese Academy of SciencesWuhan 430072，China
2. Wuhan ln~t ztute of Virology，Ch inese Academy of S~a'ence$，Wuhan 430071，China
3. Hunan Agricultural Universlty Changsha 410128．China

Abstract: Abstract：Some characterizations of four Grass Carp Reovirus(GCRV)strains were comparatively studied at the first time．It showed that the stored GCRV873，GCRVs75 and GCRv876 at minus 3012 for ten years still remained infectious to CIK cells，and the 3 virus titers could reach above 10 TCIDs0／ mI ，which was a little lower than the newly isolated GCRV99l strain s．After inoculation 3 4 genera— tions，their titers rised gradually，and the titer of GCRV873 could be up to the highest，which was al— most 6 4x10“TCIDso／mI with 0．05PFu／cellmultiplicity ofinfection(MOI)at 2812 ．Theinfec— tive ability of the four strains at different temperatures(28℃ ，3l℃ 。34℃ ，37℃ and 41℃ )was also detected，which revealed that CPE appeared in CIK cells under above conditions，but titers depressed as the temperature rised In addition，4 GCRV—ds RNAs were also compared in a same electronic con— dition。