Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus

ZHANG Yu—ping; CHEN Sheng—liang; CHEN Li—qu; Tian xian

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April 2002

ZHANG Yu—ping, CHEN Sheng—liang, CHEN Li—qu and Tian xian. Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus[J]. Virologica Sinica, 2002, 17(2): 189-192.

Citation: ZHANG Yu—ping, CHEN Sheng—liang, CHEN Li—qu, Tian xian. Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus .VIROLOGICA SINICA, 2002, 17(2) : 189-192.

人巨细胞病毒PP71基因序列分析与表达

1.
中国科学院武汉病毒研究所．湖北武汉430071

摘要

Abstract：PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR． After digestion by Barn H I and Hin dⅢ．the fragment was cloned into the high level expre~ion vec— tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E．coli BI 2l(DE3)and ln— duced by IPTG，high 1evel protein V,’as produced，and the target protein WaS 40％ of all proteins．Purifl— eatlo~ recovery rate was high and up to 92 4％ of the target protein before purification．which provides scientific basis on research of HCM V pathogenesis and diagnosis．
- H um an Cytomegalor Virus
- , PP7 gene
- , Clone
- , Protein expresion

Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus

1.
Wuhan Institute of Virology，Chinese Academy of Sciences，Wuhan 430071，China

Abstract

Abstract：PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR． After digestion by Barn H I and Hin dⅢ．the fragment was cloned into the high level expre~ion vec— tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E．coli BI 2l(DE3)and ln— duced by IPTG，high 1evel protein V,’as produced，and the target protein WaS 40％ of all proteins．Purifl— eatlo~ recovery rate was high and up to 92 4％ of the target protein before purification．which provides scientific basis on research of HCM V pathogenesis and diagnosis．
- Human Cytomegalor Virus
- , PP7 gene
- , Clone
- , Protein expresion

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus

1. Wuhan Institute of Virology，Chinese Academy of Sciences，Wuhan 430071，China

Abstract: Abstract：PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR． After digestion by Barn H I and Hin dⅢ．the fragment was cloned into the high level expre~ion vec— tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E．coli BI 2l(DE3)and ln— duced by IPTG，high 1evel protein V,’as produced，and the target protein WaS 40％ of all proteins．Purifl— eatlo~ recovery rate was high and up to 92 4％ of the target protein before purification．which provides scientific basis on research of HCM V pathogenesis and diagnosis．