Study on the Endonuelease Site of G1 Region of Hantavirus Genome

XIONG Li—juan; LUO Duan—de; ZENG Ling—lan; CAI Shu—qing

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June 2002

XIONG Li—juan, LUO Duan—de, ZENG Ling—lan and CAI Shu—qing. Study on the Endonuelease Site of G1 Region of Hantavirus Genome[J]. Virologica Sinica, 2002, 17(3): 195-197.

Citation: XIONG Li—juan, LUO Duan—de, ZENG Ling—lan, CAI Shu—qing. Study on the Endonuelease Site of G1 Region of Hantavirus Genome .VIROLOGICA SINICA, 2002, 17(3) : 195-197.

汉坦病毒基因组G1区部分酶切位点的研究

1.
华中科技大学同济医学院附属协和医院传染科，湖北武汉430022

摘要

用分型RT—PCR方法对34份武汉地区肾综合征出血热患者外周血标本扩增，扩增的目的片段为汉坦病毒 M 片段G1基因部分序列，应用Hin C 1I和Sac I两种限制性内切酶消化扩增产物，可将汉坦病毒区分为HTN型和 SEO型．结果与分型PCR有很高的一致性，并可迅速筛查在Hinc 1I和Sac I酶切位点有基因变异的变异株。
- 汉坦病毒
- , 病毒分型
- , 限制性内切酶

Study on the Endonuelease Site of G1 Region of Hantavirus Genome

1.
Department of Infectious Diseases，Union Hospital，Tong．ji Medical College， Huazhong University of Technology and Science．Wuhan 430022，China

Abstract

Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of 34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan．The products contained within G 1 gene of M segm ent， w ere digested with H in C II and Sac I respectively， thereby， han— tavirus could be typed into HTN and SEO．The results of RFLP—PCR were highly consistent with typ— ing RT—PCR，and furthermore，RFI P—PCR could quickly screen the variant strain with gene mutation at the restriction site of HiI"l C II or SacI．
- Hantavirus
- , Virus typing
- , RFLP

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the Endonuelease Site of G1 Region of Hantavirus Genome

1. Department of Infectious Diseases，Union Hospital，Tong．ji Medical College， Huazhong University of Technology and Science．Wuhan 430022，China

Abstract: Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of 34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan．The products contained within G 1 gene of M segm ent， w ere digested with H in C II and Sac I respectively， thereby， han— tavirus could be typed into HTN and SEO．The results of RFLP—PCR were highly consistent with typ— ing RT—PCR，and furthermore，RFI P—PCR could quickly screen the variant strain with gene mutation at the restriction site of HiI"l C II or SacI．