采用PCR扩增、pGEM—T载体克隆和核苷酸序列分析的方法对一例武汉地区及两例五峰县高发区宫颈癌患 者体内HPV16型的E7基因编码区进行序列分析并与野生型(德国标准株)及已发表的HPV16湖北株(HPVHB) 进行了比较。结果发现武汉地区HDV16型E7基因仅第54位出现一个同义突变，而高发区HPV16型E7基因存 在差异，第77位氨基酸由精氨酸(Arg)变为半胱氨酸(Cys)，第96位由谷氨酰氨酸(Gln)变为精氨酸(Arg)，E7蛋白 的二级结构及亲、疏水性也相应改变，与野生型有较大差异。

In order tO study the structure specificity of HPV 1 6 E 7 gene of cervical carcinoma in W uhan city and W ufeng county of Hubei the tissue DNA was abstracted from cervical carcinom a biopsies from W uhan and high incidence region of cervical carcinoma in W uhan county． HPV 16 E 7 gene was am pli— fied by PCR from the cervical carcinoma tissus DNA with the infection of HPV1 6．and then was cloned into pGEM —T vector．After sequencing the double strands，the gene was com pared with the prototype E 7 gene of HPV 16，HPV 16 Hubei strain and com pared with each other．Sequencing results showed one point mutation in the viral neocleotide sequence of high incidence region that the 229 th neocleotide “C’’was changed into “T”，which caused the Arg cordon TGT tO convert into a Cys cordon CGT ． This may be the cause of high incidence of HPV infection and high oncogenisis．The results also show that there is only a synonymous mutation in the viral neocleotide sequence of W uhan which is different from the HPV 16 HB E 7 gene，suggesting that HPV 16 HB and the HPV16 prototype are CO—exist in Hubei， but the HPV 16 prototype are the m ain type．