将汉滩病毒囊膜糖蛋白G1与核蛋白(NP)部分片段以不同方式拼接，构建G1SO．7或SO．7G1嵌合基因，分 别插入杆状病毒表达载体pFBD。转化DH10Bac致敏菌。获得含有嵌合基因的重组穿梭质粒Bacmid，用其转染Sf9 细胞．快速筛选出含有G1SO 7或SO．7G1嵌合基因的重组杆状病毒，在昆虫细胞中表达外源融合蛋白。利用间接 免疫荧光、ELISA和免疫印迹对表达产物进行检测。结果表明，含G1SO ．7嵌合基因之重组杆状病毒可在昆虫细胞 中表达出融合蛋白，该蛋白可被抗汉滩病毒核蛋白及糖蛋白G1特异性单抗所识别，其分子量约97kD；含SO．7G1 嵌合基因之重组杆状病毒在昆虫细胞中表达的融合蛋白，只能被抗汉滩病毒核蛋白特异性单抗所识别，其分子量 约43kD。上述结果提示．G1SO．7嵌合基因可能在昆虫细胞中表达出完整的具有生物学活性的融合蛋白，SO．7G1 嵌和基因的昆虫细胞表达产物不完整，且生物学活性不如G1SO ．7嵌合基因的表达产物。

H antaan virus glycoprotein G1 and nucleoprotein partial fragment w ere connected in differ— ent ways．The constructed chim eric gene G 1 SO ．7 or SO．7G 1 was inserted into baculovirus expression vector pFBD．The recombinant shuttle plasmids(Bacmids)containing chimeric genes were obtained in E ．coli DH 10Bac．Sf9 cells were transfected by the recom binant Bacm ids，and then recom binant bac— uloviruses were selected and fusion proteins were expressed in insect cells．The expression w as identified by ELISA，im m unofluorescence and W estern blot．It show es that，the recom binant baculovirus contain— ing the chim eric gene G1SO ．7 could express the fusion protein in insect cells．This protein，whose molecular weight was about 97kD，could be recognized by the hantaan virus nucleoprotein specific mAb and glycoprotein G1 specific m Ab．The fusion protein，which was expres ed by the recom binant bac— uloviurs containing the chim eric gene SO ．7G1 in insect cells，could only be recognized by the hantaan virus nucleoprotein specific mA b．Its molecular weight was about 43kD ．It suggests that the chimeric gene G 1SO ．7 can express biologically active integrated fusion protein in insect cells，while the product of SO．7G 1 isn’t integrated and its biological activity isn’t as good as the product of G1SO ．7．