Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx

WAN Yan—ping; W U Yi—mou; TANG Li—zhi; LIAO Duan—fang

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June 2002

WAN Yan—ping, W U Yi—mou, TANG Li—zhi and LIAO Duan—fang. Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx[J]. Virologica Sinica, 2002, 17(3): 230-233.

Citation: WAN Yan—ping, W U Yi—mou, TANG Li—zhi, LIAO Duan—fang. Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx .VIROLOGICA SINICA, 2002, 17(3) : 230-233.

腺病毒EIB 55kD癌蛋白与hDaxx的相互作用

1.
1南华大学基础医学院微生物学教研室，湖南衡阳，421001
2.
南华大学基础医学院药理学教研室，湖南衡阳，421001

摘要

为了探讨腺病毒(adenovirus，Ad)E1B 55kD癌蛋白(Ad E1B 55kD)打破hDaxx和PML共定位细胞核的作用机制，本文利用体内外共免疫沉淀反应研究Ad EIB 55kD与hDaxx的结合反应，并通过酵母双杂交体系测定两种蛋白质的相互作用及其作用的氨基酸残基序列。结果显示：Ad2 EIB 55kD 通过C端58个氨基酸(aa)与hDaxx结合并发生相互作用。Adl2 EIB 55kD与hDaxx结合需全序列aa及其构象。共免疫沉淀反应和Western blot结果证实Ad2／5或Adl2 EIB 55kD能在体内外与hDaxx直接结合。
- 腺病毒EIB 55tO 癌蛋白
- , hDaxx
- , 酵母双杂交测定
- , 相互作用

Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx

1.
1．Departmerit ofM icrobiology，School of Basic Medicine，Nanhua University，Hengyang 421001。China： 2．Departmerit of Pharmacology，Schol of Basic Medicine，Nanhua University，Hengyang 421001，China

Abstract

Abstract：To question the reason of CO—localization of hDaxx and PM L in PODs disrupted by Aden— ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD)，the interaction of Ad E1 B 55kD with hDaxx was analyzed by the yeast two-hybrid assay， and the direct binding of Ad E1 B 55kD with hDaxx was studied by coimm unoprecipitation reaction in vivo or in vitro．The results show that Ad E1B 55kD oncoproteins interact with hDaxx by binding the C-terminus 58 am ino acids of A d2 E1B 55kD or the full length of Adl2 E1B 55kD．Ad2／5 or Adl2 E1B 55kD directly binds hDaxx in vivo or in vitro．
- Ad E1B oncoproteins
- , hDaxx
- , Yeast two—hybrid assay
- , Interaction

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx

1. 1．Departmerit ofM icrobiology，School of Basic Medicine，Nanhua University，Hengyang 421001。China： 2．Departmerit of Pharmacology，Schol of Basic Medicine，Nanhua University，Hengyang 421001，China

Abstract: Abstract：To question the reason of CO—localization of hDaxx and PM L in PODs disrupted by Aden— ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD)，the interaction of Ad E1 B 55kD with hDaxx was analyzed by the yeast two-hybrid assay， and the direct binding of Ad E1 B 55kD with hDaxx was studied by coimm unoprecipitation reaction in vivo or in vitro．The results show that Ad E1B 55kD oncoproteins interact with hDaxx by binding the C-terminus 58 am ino acids of A d2 E1B 55kD or the full length of Adl2 E1B 55kD．Ad2／5 or Adl2 E1B 55kD directly binds hDaxx in vivo or in vitro．