WU Xing—quan, W U Zu—j ian, XIE Lian—hui“ and I IN Qi—ying. The Clone and Expression of the Coat Protein Gene of Potato Virus S in E .coli[J]. Virologica Sinica, 2002, 17(3): 248-251.
Citation: WU Xing—quan, W U Zu—j ian, XIE Lian—hui“, I IN Qi—ying. The Clone and Expression of the Coat Protein Gene of Potato Virus S in E .coli .VIROLOGICA SINICA, 2002, 17(3) : 248-251.

马铃薯s病毒外壳蛋白基因的克隆与原核表达

  • 依据马铃薯S病毒(Potato virusS,PVS)外壳蛋白(CP)基因序列(885bp)设计合成了两对引物,通过RT—PCR 扩增得到长0.8kb的目的片段,将目的片段转入大肠杆菌,酶切鉴定证明得到了含有目的片段的重组子,测定序列 结果与其他PVS分离物CP基因的序列比较,发现其核苷酸同源性达95% 左右;构建了含PVS CP基因的融合蛋 白原核表达载体,并在大肠杆菌中得到表达,SDS-PAGE测定融合蛋白的分子量为58kD。

The Clone and Expression of the Coat Protein Gene of Potato Virus S in E .coli

  • Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de— signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR.The gene was cloned into pGEX一2T vector and then transformed into E .coli DH5a.The gene has high homolo— gy(95% )comparing with other isolate genes.The expression vector p2TSCP was constructed.The coat protein gene was expressed in E .coli DH5a and a 58kD protein was analysed by SDS—PAGE.

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    The Clone and Expression of the Coat Protein Gene of Potato Virus S in E .coli

    • 1. Plant Virus Institute of F ian Agriculture and Forestry University,Fuzhou 350008,China

    Abstract: Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de— signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR.The gene was cloned into pGEX一2T vector and then transformed into E .coli DH5a.The gene has high homolo— gy(95% )comparing with other isolate genes.The expression vector p2TSCP was constructed.The coat protein gene was expressed in E .coli DH5a and a 58kD protein was analysed by SDS—PAGE.

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