Identification and Characterization of the RNase lit Gene of the Tiger Frog Virus

LU Ling; HE Jian—guo; HE Hau—hong; DENG Min; WENG Shao-ping; WANG Xiao-hong

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June 2002

LU Ling, HE Jian—guo, HE Hau—hong, DENG Min, WENG Shao-ping and WANG Xiao-hong. Identification and Characterization of the RNase lit Gene of the Tiger Frog Virus[J]. Virologica Sinica, 2002, 17(3): 257-262.

Citation: LU Ling, HE Jian—guo, HE Hau—hong, DENG Min, WENG Shao-ping, WANG Xiao-hong. Identification and Characterization of the RNase lit Gene of the Tiger Frog Virus .VIROLOGICA SINICA, 2002, 17(3) : 257-262.

蛙病毒核糖核酸酶Ⅲ基因结构及其序列分析

1.
中山大学生命科学学院，广东广州510275

摘要

对蛙病毒(TFv)核糖核酸酶Ⅲ基因序列进行分析。TFv 基因组中含有完整的核糖核酸酶Ⅲ 基因序列，全长为1 ll3bp，GC含量为56．63％。其推定蛋白质的分子量为40．47kD，等电点为7．99。序列结构分析发现在编码区的下游有可形成茎环的反向重复序列和形成发夹结构的回文序列。与其它物种相比，TFv与虹彩病毒的LCDV．1 和CIV的核糖核酸酶Ⅲ基因的氨基酸序列同源性较高，与酵母、线虫等物种的相应基因的同源性较低。
- 蛙病毒(TFv)
- , 核糖核酸酶IlI
- , 序列分析

Identification and Characterization of the RNase lit Gene of the Tiger Frog Virus

1.
School of life sciences，Zhongshan University，Guangzhou 510275

Abstract

From the tiger frog virus(TFV)genome，an open reading frame(ORF)，whose deduced amino acid sequence(aa)shows identity with RNase 111。was identified．The ORF has 1 113bp long and codes a putative protein of 371 aa with a predicted molecular mass of 40．47kD and a G+ C content of 56．63％． Stem loops and short dyad sequences appear in the downstream of the translation stop codon．Compared with the RNase 111 S found in TFV and other species．the higher homology among TFV and the tWO iridovirus is present，and TFV has lower identity with other species， especially yeast and rhabditida．
- TFV
- , RNase III
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification and Characterization of the RNase lit Gene of the Tiger Frog Virus

1. School of life sciences，Zhongshan University，Guangzhou 510275

Abstract: From the tiger frog virus(TFV)genome，an open reading frame(ORF)，whose deduced amino acid sequence(aa)shows identity with RNase 111。was identified．The ORF has 1 113bp long and codes a putative protein of 371 aa with a predicted molecular mass of 40．47kD and a G+ C content of 56．63％． Stem loops and short dyad sequences appear in the downstream of the translation stop codon．Compared with the RNase 111 S found in TFV and other species．the higher homology among TFV and the tWO iridovirus is present，and TFV has lower identity with other species， especially yeast and rhabditida．