Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation

DING Yong; W U; ZHANG W; ZHAO M; TAN Yun

PDF
Cite
Share

facebook

twitter

google

linkedin

August 2002

DING Yong, W U, ZHANG W, ZHAO M and TAN Yun. Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation[J]. Virologica Sinica, 2002, 17(4): 304-307.

Citation: DING Yong, W U, ZHANG W, ZHAO M, TAN Yun. Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation .VIROLOGICA SINICA, 2002, 17(4) : 304-307.

重组人乳头瘤病毒核酸疫苗诱导特异性淋巴细胞增殖

伍欣星 ,

1.
1．湖北省卫生职工医学院生化教研室，湖北荆州434000
2.
武汉大学医学院分子生物学研究室．湖北武汉430071

摘要

采用分子克隆技术，将人乳头瘤病毒16型E7(HPV 16 E7)基因重组于真核表达载体上，构建了HPV 16 E7 核酸疫苗。将该疫苗通过皮内注射方式免疫BNb／c纯系小鼠。基因免疫后制备小鼠脾淋巴细胞悬液，经体外E7 蛋白再次刺激后用M1vr比色法检测出了特异性淋巴细胞增殖反应。由于特异性淋巴细胞增殖是反映细胞免疫功能的简便有效方法，所以本实验表明HPV 16 E7核酸疫苗构建正确，并能够诱导机体产生特异性细胞免疫应答。
- 人乳头瘤病毒
- , 核酸疫苗
- , 基因免疫
- , 淋巴细胞增殖

Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation

1.
1．Hubei Medical stuff College，Jingzhou 434000，China
2.
Medical College，Wuhan University，Wuhan 430071，China

Abstract

Using molecular cloning technique，HPV16 E7 gene was cloned into eukarytic expression vector．HPV16 E7 DNA vaccine was constructed and Balb／c mice were immunized by intradermal ad— ministration with HPV16 E7 vaccine．After immunization，mice spleen lymphocytes were prepared and restimulated by E7 protein in vitro．Specific lympproliferation were detected by MTT colorimetric as— say．Because detection of specific lymphoproliferation is a simple and efficient way，which can reflect cell—mediated immunity．The study showed that the construction of HPV16 E7 vaccine was correct．It can induce a specific cell—mediated immunization
- Human papillomavirus(HPV)
- , DNA vaccine
- , Gene immunization
- , Lymphoproliferation

References
Proportional views

PDF

Article Metrics

Article views(4029) PDF downloads(743) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation

1. 1．Hubei Medical stuff College，Jingzhou 434000，China
2. Medical College，Wuhan University，Wuhan 430071，China

Abstract: Using molecular cloning technique，HPV16 E7 gene was cloned into eukarytic expression vector．HPV16 E7 DNA vaccine was constructed and Balb／c mice were immunized by intradermal ad— ministration with HPV16 E7 vaccine．After immunization，mice spleen lymphocytes were prepared and restimulated by E7 protein in vitro．Specific lympproliferation were detected by MTT colorimetric as— say．Because detection of specific lymphoproliferation is a simple and efficient way，which can reflect cell—mediated immunity．The study showed that the construction of HPV16 E7 vaccine was correct．It can induce a specific cell—mediated immunization