为了评价重组大肠杆菌表达的HPV16L1蛋白和重组腺病毒表达的HPV16L1一VLP两种抗原在检测宫颈癌 抗16 L1或VLP抗体及在宫颈癌血清学诊断意义上的差别，应用PCR技术从宫颈癌组织的DNA中扩增出全长 1535bp的HPV16L1基因片段，克隆至pUC18一T载体中，进行DNA测序鉴定。然后，将HPV16L1基因克隆至 pGEX-2T表达载体中，并诱导表达HPV16L1融合蛋白，分子量为83kD，能被HPV16L1单克隆抗体所识别。经 GST柱层析法纯化后，与重组腺病毒表达的HPV16L1一VLP分别经酶联免疫吸附(ELISA)法检测12份宫颈癌患者 和35份献血员血清。12例宫颈癌血清标本中，抗HPV16L1蛋白的抗体阳性率为7例(占58．3％)；抗HPV16L1一 VLP的抗体阳性率为8例(占66．7％)。经大肠杆菌表达的重组抗原HPV16L1检测为HPV16抗体IgG(+)的7 份患者血清。利用HPV16L1一VLP试剂盒检测均阳性；经大肠杆菌表达的重组抗原检测为HPV16抗体IgG(一)的5份患者血清，利用HPV16L1．VLP试剂盒检测有1份阳性。两者对HPV16抗体的阳性检出率并无显著差异(P0．05)。本实验结果说明HPV16与宫颈癌高度相关，利用大肠杆菌表达的重组抗原HPV16L1和HPV16L1一VLP重组抗原检测抗体的敏感性并不受影响。利用重组抗原HPV16 L1对宫颈癌的抗体进行定性、定量分析有助于该疾病的诊断。

In order to use major capsid protein L1 of Human papillomaviruses(HPV)16 produced in a fused fom in E ．coli and HPV16 L1 VLP produced in recombinant adenovirus in 293 cells as antigen to detecton antibodies of HPV 1 6 L1 in the cervical cancer people．and compare the serological differ— enee of the two antigen in the diagnosis of cervical cancer，we used PCR to amplify HPV1 6L1 gene from the cervical cancer， then cloned into pUC18一T． After DNA sequencing， HPV16L1 gene was cloned into pGEX一2T expressing vector，and induced by IPTG to express in E ．coli as glutathione—S— transferase—L1(GST—LI)fusions and purified to near homogeneity as antigen，together with HPV16 L1 VLP produced in recombinant adenovirus in 293 cells，to test antibo dies of human—papillomavirus (HPV)16 L1 of 12 cervical cancer and 53 blood donors．The gene derived from the cervical cancer HPV16 genome was 1535 bp in length，and expressed by E ．coli to the full—length 83 kD polypep— tide，which recognized 1)y HPV16L1 monocloned antibody．In the 12 cervical cancer sera，there were 7 positive in HPV16L1 antibody(58．3％ )；while 8 positive in HPV16L1 VLP antibody(66．7％)．A— mong 7 positive in anti—HPV16 L1using HPV16L1 protein from the E ．coli as antigen，all are positive when using HPV 16L1一V LP as antigen．W hile among 5 negative in anti—HPV16 Llusing HPV 16L1 protein from the E ．coli as antigen， there is 1 positive when using HPV1 6L1一VI P as antigen．There are no difference between two group as antigens in ELISA detection．(P0．05)Our research sug． gested that HPV 1 6 is highly associated with cervical cancer．The sensitivity of the test is the same whether using HPV 16I 1 protein from the E ． coli or HPV16L1一VLP as antigens． To detect HPV16I 1 antibody is helpful to diagnosis cervical cancer．