CHEN Jiong.CHEN Jian—ping and Phylogenetic Tree Analysis of UTR and Transmembrane Structure Prediction of Proteins of Genus Bymovirus[J]. Virologica Sinica, 2002, 17(4): 344-349.
Citation: CHEN Jiong.CHEN Jian—ping, . Phylogenetic Tree Analysis of UTR and Transmembrane Structure Prediction of Proteins of Genus Bymovirus .VIROLOGICA SINICA, 2002, 17(4) : 344-349.

大麦黄花叶病毒属成员UTR系统进化树分析及编码蛋白跨膜结构推测

  • 对大麦黄花叶病毒属成员的同源性和系统进化树分析表明,同一病毒RNA1和2基因组5’-UTR区域在进化 上的相关性高于不同病毒同一RNA组分间的关系,而3’-UTR则相反。蛋白质二级结构分析显示RNA1编码的 P3和14K 蛋白存在跨膜结构。BaMMV 6 1分离物P3蛋白跨膜结构在大肠杆菌中原核表达时有致死作用。类 比P0tyviridae科其它属成员功能,此2个蛋白可能与膜附着功能相关。RNA2编码的P2蛋白存在两个结构保守的 跨膜区域,一些摩擦接种保存的大麦和性花叶病毒(BaMMV)分离物和燕麦花叶病毒(OMV)P2蛋白此两个结构 (或一个)缺失后,不能由介体禾谷多粘菌(P.graminis)传播,揭示P2蛋白跨膜结构与禾谷多粘菌传播能力相关。

Phylogenetic Tree Analysis of UTR and Transmembrane Structure Prediction of Proteins of Genus Bymovirus

  • Homology and phylogenetic tree analysis of genus Bymovirus suggested that the 5 一UTR of RNA1 and RNA2 of the same virus were more similar than those of the same RNAs of different virus— es.but the instance of 3 UTR was opposite.Secondary structure analysis on polypoteins showed that P3 and 14K encoded by RNA1 contained transmembrane(TM)stuctures.TM region in P3 protein of BaM MV ALS1 isolates was toxic to the E .coli.By analogy to other members of family Potyviridae. these structures were predicted to have the function as attachment to the membranes.P2 protein en— coded by RNA2 also contained two TM regions.Some BaMMV isolates which were maintained me— chanically for a long period of times occurred deletions in these regions and were not transmitted by Polymyxa graminis.It was suggested that the TM structures were closely related to vector transmis— sion.

  • 加载中
  • 加载中

Article Metrics

Article views(4069) PDF downloads(789) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Phylogenetic Tree Analysis of UTR and Transmembrane Structure Prediction of Proteins of Genus Bymovirus

    • 1. 1.Virology Laboratory,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
    • 2. College of Life Sciences,Zhqiang University,Hangzhou 310029,China

    Abstract: Homology and phylogenetic tree analysis of genus Bymovirus suggested that the 5 一UTR of RNA1 and RNA2 of the same virus were more similar than those of the same RNAs of different virus— es.but the instance of 3 UTR was opposite.Secondary structure analysis on polypoteins showed that P3 and 14K encoded by RNA1 contained transmembrane(TM)stuctures.TM region in P3 protein of BaM MV ALS1 isolates was toxic to the E .coli.By analogy to other members of family Potyviridae. these structures were predicted to have the function as attachment to the membranes.P2 protein en— coded by RNA2 also contained two TM regions.Some BaMMV isolates which were maintained me— chanically for a long period of times occurred deletions in these regions and were not transmitted by Polymyxa graminis.It was suggested that the TM structures were closely related to vector transmis— sion.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return