Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus

FU Ming—jia; WU Zu—jian; LIN Qi—ying’’; XIE Lian—hui

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August 2002

FU Ming—jia, WU Zu—jian, LIN Qi—ying’’ and XIE Lian—hui. Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus[J]. Virologica Sinica, 2002, 17(4): 350-353.

Citation: FU Ming—jia, WU Zu—jian, LIN Qi—ying’’, XIE Lian—hui. Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus .VIROLOGICA SINICA, 2002, 17(4) : 350-353.

榆黄蘑中一种抗病毒蛋白的纯化及其抗TMV和HBV的活性

1.
福建农林大学植物病毒研究所，福建福州350002

摘要

采用阴离子交换层析和凝胶层析方法从新鲜食用菌榆黄蘑(Plearotus citrinopileatus)中进行了抗病毒蛋白的纯化．结果获得了一个纯化蛋白YP46—46，经SDS-PAGE可确定其分子量为27．41d3。以半叶法在枯斑寄主心叶烟上检测该蛋白对烟草花叶病毒(TMV)的抑制率，发现有较好的抗TMV活性，其抑制TMV的中浓度为0．24~g／ rnL。同时以HeDG2．2．2．15细胞株为模型，对所获得的蛋白进行体外抗乙型肝炎病毒效果的评价。结果表明该蛋白对HBsAg的50％抑制时的浓度为0．08 g／mL，但对HBeAg效果不大。
- 抗病毒蛋白
- , 烟草花叶病毒
- , 乙型肝炎病毒

Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus

1.
Institute of Plant Virology，Fujian Agriculture and Forestry University，Fuzhou 350002，China

Abstract

A antiviral protein。YP46—46，was isolated and purified to homogeneity from the mushroom， Plearotus citrinopileatus．by precipitation of 40％一60％ saturation of ammonium sulfate followed by DEAE-sepharose FF ion—exchange column chromatography。 Sephacryl M S一200 High Resolution molecular sieve chromatography．The protein has a molecular weight of about 27．4kD by SDS-PAGE． The concentration of the protein was 0．24／~g／mL when the inhibition rate against Tobacco mosaic irus was 50％．Using an assay system based on HepG2．2．2．1 5 cell line，YP46—46 was studied for its inhibitory ability against Hepatitis B virus．The result shows that the concentration of 50％ inhibition of HBsAg was 0．08ttg／mL，but low effect on HBeAg．
- Antiviral protein
- , Tobacco mosaic virus
- , Hepatitis B virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus

1. Institute of Plant Virology，Fujian Agriculture and Forestry University，Fuzhou 350002，China

Abstract: A antiviral protein。YP46—46，was isolated and purified to homogeneity from the mushroom， Plearotus citrinopileatus．by precipitation of 40％一60％ saturation of ammonium sulfate followed by DEAE-sepharose FF ion—exchange column chromatography。 Sephacryl M S一200 High Resolution molecular sieve chromatography．The protein has a molecular weight of about 27．4kD by SDS-PAGE． The concentration of the protein was 0．24／~g／mL when the inhibition rate against Tobacco mosaic irus was 50％．Using an assay system based on HepG2．2．2．1 5 cell line，YP46—46 was studied for its inhibitory ability against Hepatitis B virus．The result shows that the concentration of 50％ inhibition of HBsAg was 0．08ttg／mL，but low effect on HBeAg．