New Methods for Determining Induction Rate of Prophage in Lysogenic Bacteria

FAN Cheng—peng; FANG Cheng—xiang; ZHANG Luo-zhen; DAI Shu—xiang

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August 2002

FAN Cheng—peng, FANG Cheng—xiang, ZHANG Luo-zhen and DAI Shu—xiang. New Methods for Determining Induction Rate of Prophage in Lysogenic Bacteria[J]. Virologica Sinica, 2002, 17(4): 367-370.

Citation: FAN Cheng—peng, FANG Cheng—xiang, ZHANG Luo-zhen, DAI Shu—xiang. New Methods for Determining Induction Rate of Prophage in Lysogenic Bacteria .VIROLOGICA SINICA, 2002, 17(4) : 367-370.

测定λ原噬菌体诱导频率的新方法

1.
武汉大学生命科学院,湖北武汉430072

摘要

本文报道两种测定原噬菌体紫外诱导频率的新方法一菌落计数法和平板诱导法。将溶源菌液经紫外诱导暗培养稀释后直接涂布在平板上培养，根据平板上菌落形成单位数计算噬菌体紫外诱导频率。另一种是将溶源菌与指示菌混合制备的平板用紫外线诱导，根据平板上噬菌体形成单位确定噬菌体紫外诱导频率。这两种方法不仅能准确测定噬菌体紫外诱导频率，而且操作简便，节省时间和用具，重复性好。本研究还将冬虫夏草浸出汁与溶源菌?昆合后进行紫外辐射，通过几种方法进行比较，结果证明建立的新方法确实可行，易操作；同时也表明冬虫夏草具有较强的抗紫外辐射作用。
- λ原噬菌体
- , 诱导频率
- , 软琼脂平板法
- , 菌落计数法
- , 平板诱导法

New Methods for Determining Induction Rate of Prophage in Lysogenic Bacteria

1.
College of Life Sciences，Wuhan University，Wuhan 430072，China

Abstract

Two new methods，colony counting method and plate inducing method，were established for determining ultraviolet induction rate of prophage in the present study．Culture of lysogenic bacteria (λ)which was induced hy ultraviolet irradiation and cultivated in dark was directly spread on the plate，and induction rate of prophage was calculated based on the CFUs(colony form ing units)．The plate with mixture of lysogenic bacteria and indicator strain was induced by ultraviolet irradiation．The induction rate of prophage was calculated based on the PFUs(plaque form ing units)．These two methods not only can determ ine precisely induction frequency of prophage。but also are easy tO ma— nipulate ，and they can save efforts and apparatus，they are also easy to repeat．Furthermore，the m ix— ture of Cordyceps sinensis extract and lysogenic bacteria was irradiated with U V ．The results demon— strate that the new methods are practical and easy tO conduct，and that Cordyceps sinensis has a strong protection effect against U V—irradiation．
- Induction rate of prophage
- , Soft agar layer technique
- , Colony—counting method
- , Plate—inducing method

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

New Methods for Determining Induction Rate of Prophage in Lysogenic Bacteria

1. College of Life Sciences，Wuhan University，Wuhan 430072，China

Abstract: Two new methods，colony counting method and plate inducing method，were established for determining ultraviolet induction rate of prophage in the present study．Culture of lysogenic bacteria (λ)which was induced hy ultraviolet irradiation and cultivated in dark was directly spread on the plate，and induction rate of prophage was calculated based on the CFUs(colony form ing units)．The plate with mixture of lysogenic bacteria and indicator strain was induced by ultraviolet irradiation．The induction rate of prophage was calculated based on the PFUs(plaque form ing units)．These two methods not only can determ ine precisely induction frequency of prophage。but also are easy tO ma— nipulate ，and they can save efforts and apparatus，they are also easy to repeat．Furthermore，the m ix— ture of Cordyceps sinensis extract and lysogenic bacteria was irradiated with U V ．The results demon— strate that the new methods are practical and easy tO conduct，and that Cordyceps sinensis has a strong protection effect against U V—irradiation．