Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

CHENG Zi—qiang; ZHAO Zhen—hua; HAO Yong—qing; ZHAO Xin—li; H ASI Agual

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August 2002

CHENG Zi—qiang, ZHAO Zhen—hua, HAO Yong—qing, ZHAO Xin—li and H ASI Agual. Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis[J]. Virologica Sinica, 2002, 17(4): 371-373.

Citation: CHENG Zi—qiang, ZHAO Zhen—hua, HAO Yong—qing, ZHAO Xin—li, H ASI Agual. Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis .VIROLOGICA SINICA, 2002, 17(4) : 371-373.

禽白血病病毒J亚群(ALV—J)的血清学调查及PCR诊断

1.
1．山东农业大学动物科技学院．山东泰安271018
2.
内蒙古农业大学动物科学与医学学院，内蒙古呼和浩特0100183．内蒙古自治区兽医站化验室，内蒙古呼和浩特010020
3.
内蒙古大学生物工程中心，内蒙古呼和浩特010025

摘要

禽骨髓细胞性白血病(myeloid leucosis)(或称禽骨髓细胞瘤，myelcytomatomatosis)(ML)是由禽白血病病毒(Avian leukosis virus)J亚群(ALV—J)引起的禽的一种肿瘤性传染病u J，ALV —J是英国的Payne于1991年从肉鸡中分离出来的一个新的囊膜亚群[ ，。对AI v—J的原型株，HPRS一103的致病性和传播的研究中发现，本病毒能诱导肉鸡产生骨髓细胞瘤病(ML)、肾瘤和其它多种肿瘤，死亡率为1％～2％，偶尔可高达20％。由于本病毒为AI V和禽内源性反转录病毒囊膜(E51)的重组体．5 J，因此其可通过水平传播和垂直传播迅速地感染整个鸡群，使鸡群在短时间内遭受灭顶之灾。近十年来，在许多国家，包括美国在内的肉鸡中，MI已经是引起死亡和其它生产性问题的严重原因。
- 禽骨髓细胞瘤病
- , 血清学调查
- , PCR诊断

Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

1.
1．Institute ofAnimal Science and Animal Medicine，ShanDong Agriculture University，TaiAn 271018
2.
Institute of Animal Science and Animal Medicine，Inner Mongolia Agriculture University，Huhhot 010018，China
3.
Examination Croup of Inner Mongolia Veterinary Station，Huhhot 010020

Abstract

We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region．The result indicated that the meat—type flocks of three dif—ferent farms infection rate'．of ALV—J were 28．12％(9／32)、9．38％(3／32)and 8．33％(10／12)，respec—tively，and all laying chickens infection rate were 0％(0／35，0／10)．We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test．The result indicated that6 chickens of ALV—J antibody positive were all PCR positive，and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative．The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens．
- Avian myelocytom atosis
- , Serology investigation
- , PCR diagnosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

1. 1．Institute ofAnimal Science and Animal Medicine，ShanDong Agriculture University，TaiAn 271018
2. Institute of Animal Science and Animal Medicine，Inner Mongolia Agriculture University，Huhhot 010018，China
3. Examination Croup of Inner Mongolia Veterinary Station，Huhhot 010020

Abstract: We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region．The result indicated that the meat—type flocks of three dif—ferent farms infection rate'．of ALV—J were 28．12％(9／32)、9．38％(3／32)and 8．33％(10／12)，respec—tively，and all laying chickens infection rate were 0％(0／35，0／10)．We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test．The result indicated that6 chickens of ALV—J antibody positive were all PCR positive，and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative．The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens．