CHENG Zi—qiang, ZHAO Zhen—hua, HAO Yong—qing, ZHAO Xin—li and H ASI Agual. Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis[J]. Virologica Sinica, 2002, 17(4): 371-373.
Citation:
CHENG Zi—qiang, ZHAO Zhen—hua, HAO Yong—qing, ZHAO Xin—li, H ASI Agual.
Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis .VIROLOGICA SINICA, 2002, 17(4)
: 371-373.
禽白血病病毒J亚群(ALV—J)的血清学调查及PCR诊断
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1.
1.山东农业大学动物科技学院.山东泰安271018
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2.
内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特0100183.内蒙古自治区兽医站化验室,内蒙古呼和浩特010020
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3.
内蒙古大学生物工程中心,内蒙古呼和浩特010025
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摘要
禽骨髓细胞性白血病(myeloid leucosis)(或称禽骨髓细胞瘤,myelcytomatomatosis)(ML)是由禽白血病病毒(Avian leukosis virus)J亚群(ALV—J)引起的禽的一种肿瘤性传染病u J,ALV —J是英国的Payne于1991年从肉鸡中分离出来的一个新的囊膜亚群[ , 。对AI v—J的原型株,HPRS一103的致病性和传播的研究中发现,本病毒能诱导肉鸡产生骨髓细胞瘤病(ML)、肾瘤和其它多种肿瘤,死亡率为1% ~2%,偶尔可高达20%。由于本病毒为AI V和禽内源性反转录病毒囊膜(E51)的重组体 .5 J,因此其可通过水平传播和垂直传播迅速地感染整个鸡群,使鸡群在短时间内遭受灭顶之灾。近十年来,在许多国家,包括美国在内的肉鸡中,MI已经是引起死亡和其它生产性问题的严重原因。
Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis
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1.Institute ofAnimal Science and Animal Medicine,ShanDong Agriculture University,TaiAn 271018
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Institute of Animal Science and Animal Medicine,Inner Mongolia Agriculture University,Huhhot 010018,China
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Examination Croup of Inner Mongolia Veterinary Station,Huhhot 010020
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Abstract
We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region.The result indicated that the meat—type flocks of three dif—ferent farms infection rate'.of ALV—J were 28.12%(9/32)、9.38%(3/32)and 8.33%(10/12),respec—tively,and all laying chickens infection rate were 0%(0/35,0/10).We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test.The result indicated that6 chickens of ALV—J antibody positive were all PCR positive,and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative.The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens.
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References
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Proportional views
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