Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae)

CHENG Kai; WANG Chun—yan; GUO Ya—xin; SHI Zheng—li 。ZHAO Yi—jun

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August 2002

CHENG Kai, WANG Chun—yan, GUO Ya—xin, SHI Zheng—li 。ZHAO Yi—jun and Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae)[J]. Virologica Sinica, 2002, 17(4): 374-376.

Citation: CHENG Kai, WANG Chun—yan, GUO Ya—xin, SHI Zheng—li 。ZHAO Yi—jun, . Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae) .VIROLOGICA SINICA, 2002, 17(4) : 374-376.

感染丝状蓝藻的噬藻体的裂解周期和释放量的测定

1.
1．华中师范大学生命科学学院，湖北武汉430079
2.
中国科学院武汉病毒研究所无脊椎动物病毒学联合开放实验室，湖北武汉430071

摘要

近年来，随着浮游病毒的认识的深入，人们认识到浮游病毒对水体中初级生产力的影响是巨大的ll J，其主要证据就是发现噬藻体在海洋蓝藻的种群控制上发挥着重要作用-2 J。噬藻体的释放量和裂解周期是衡量噬藻体感染力的重要指标，很多重要的生态指标如病毒在生态系统中对宿主的致死率、病毒种群得以维持的阈浓度等都需要使用病毒的释放量和裂解周期来加以推算l3· ，因此准确地测定这两个基本参数是十分重要的。在自然界，很多丝状蓝藻，如颤藻、鱼腥藻、螺旋藻、席藻等是能够形成水华的，其中有些还具有产毒的功能l5 J。丝状蓝藻的形态特征有别于单细胞蓝藻，在被噬藻体感染时，丝状蓝藻的感染周期和光合生理也与单细胞蓝藻有较大的差异l6 J，因此研究裂解丝状蓝藻的噬藻体的方法可能不同于感染单细胞的噬藻体。本次试验以一种感染丝状宿主的噬藻体为材料，探讨了确定其裂解周期和释放量的研究方法。
- 噬藻体
- , 丝状蓝藻
- , 裂解周期
- , 释放量

Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae)

1.
1．College ofLife Science，Centeral China Normal University，Wuhan 430079，China
2.
Joint Laboratory of Invertebrate Virology，Wuhan Institute of Virology，the Chinese Academy of Sciences．Wuhan 43007 1．China

Abstract

Several methods were t~ted for measurement of lysing cycle and burst size of the cyanophage infecting the ilia— mentous cyanobacterium—Plectonema boryanum IU594．The rm~ts indicated that the lysing curve of host cells was con— sistent with the one-step growth curve of cyanophage，thus it was more convenient tO substitute measuring the lysing curve of host cells for the one—step growth curve measurement of cyanophage．Besides，the burst size increased with the re— ducing cyanophage inoculation．reaching the maxium of 206PFU／Cel by 1 PFU infection．It was suggested that more ex— act burst size of cyanophage could be obtained under 1PFU cyanophage inoculation．
- Cyanophage
- , Filamentous cyanobacteria
- , Lysing cycle

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae)

1. 1．College ofLife Science，Centeral China Normal University，Wuhan 430079，China
2. Joint Laboratory of Invertebrate Virology，Wuhan Institute of Virology，the Chinese Academy of Sciences．Wuhan 43007 1．China

Abstract: Several methods were t~ted for measurement of lysing cycle and burst size of the cyanophage infecting the ilia— mentous cyanobacterium—Plectonema boryanum IU594．The rm~ts indicated that the lysing curve of host cells was con— sistent with the one-step growth curve of cyanophage，thus it was more convenient tO substitute measuring the lysing curve of host cells for the one—step growth curve measurement of cyanophage．Besides，the burst size increased with the re— ducing cyanophage inoculation．reaching the maxium of 206PFU／Cel by 1 PFU infection．It was suggested that more ex— act burst size of cyanophage could be obtained under 1PFU cyanophage inoculation．