ZHANG Fang—lin, XU Zhi—kai, LUO Wen, YAN Yan, WU Xing—an, LIU Yong, BAI Wen—tao, ZHAO Qian and WANG Hai—ta. Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays[J]. Virologica Sinica, 2003, 18(1): 14-17.
Citation: ZHANG Fang—lin, XU Zhi—kai, LUO Wen, YAN Yan, WU Xing—an, LIU Yong, BAI Wen—tao, ZHAO Qian, WANG Hai—ta. Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays .VIROLOGICA SINICA, 2003, 18(1) : 14-17.

汉滩病毒M 和S基因不同拼接方式嵌合基因免疫效果的研究

  • 摘要:本文在前期工作的基础上,构建了汉滩病毒76一l18株M 基因G2片段与S基因5 端0.7Kb片段的嵌合基 因真核表达载体pcDNA3.1一G2s0.7及pcDNA3.1一SO.7G2;用该质粒免疫BALB,c小鼠,结果表明两种质粒免疫小 鼠可同时诱导产生抗汉滩病毒核蛋白(NP)及糖蛋白(GP)特异性的抗体,且前者刺激产生的抗体效价明显高 于后者。淋巴细胞增殖实验表明,pcDNA3.1一G2s0.7组免疫小鼠脾细胞时NP及GP的增殖指数均明显高于空载 体对照组,而pcDNA3.卜SO .7G2组未检测到其淋巴细胞有明显的增殖。这说明汉滩病毒M基因G2片段及S基因 0.7Kb片段的嵌合基因既可刺激机体产生特异的抗汉滩病毒体液免疫应答,也可刺激机体产生特异的细胞免疫应 答。不同拼接方式对嵌合基因免疫效果有很大影响,嵌合基因G2SO.7这种拼接方式明显优于SO.7G2。

Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays

  • Abstract:Recombinant euckaryotic expression vectors pcDNA3.1二G2S0.7 and pcDNA3.1一S0.7G2 were constructed by cloning chimeric genes containing G2 fragment of M segm ent and 0.7Kb fragment of S segment into pcDNA3.1 (+、. Then BALB/c mice were vaccinated by the two vectors. EUSA results showed that both the vectors could induce specific antibodies against NP and GP.The specific antibody titers stimulated by pcDNA3.1一G2S0.7 were obviously higher、that that of pcDNA3.1一S0.7G2. M IT re— sults showed that the stimulation indexes of splenocytes of pcDNA3.1一G2S0.7 to NP and GP were sig— nificantly higher than that of contro1.However.that of pcDNA3.1一SO.7G2 were equal to that of contro1. It is suggested that the chimeric genes of Hantaan virus containing G2 fragment of M segm ent and 0.7Kb fragment of S segm ent could directly specific anti—Hantaan virus humoral immunity and cellular immunity in BALB/c mice.Th e diferent splicing ways could afect the immunity of chimeric genes.

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    Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays

    • 1. Department ofMicrobiology,而urth Military Medical University,Xi’nn 710032,China

    Abstract: Abstract:Recombinant euckaryotic expression vectors pcDNA3.1二G2S0.7 and pcDNA3.1一S0.7G2 were constructed by cloning chimeric genes containing G2 fragment of M segm ent and 0.7Kb fragment of S segment into pcDNA3.1 (+、. Then BALB/c mice were vaccinated by the two vectors. EUSA results showed that both the vectors could induce specific antibodies against NP and GP.The specific antibody titers stimulated by pcDNA3.1一G2S0.7 were obviously higher、that that of pcDNA3.1一S0.7G2. M IT re— sults showed that the stimulation indexes of splenocytes of pcDNA3.1一G2S0.7 to NP and GP were sig— nificantly higher than that of contro1.However.that of pcDNA3.1一SO.7G2 were equal to that of contro1. It is suggested that the chimeric genes of Hantaan virus containing G2 fragment of M segm ent and 0.7Kb fragment of S segm ent could directly specific anti—Hantaan virus humoral immunity and cellular immunity in BALB/c mice.Th e diferent splicing ways could afect the immunity of chimeric genes.

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