HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection

ZHANG Ying; BAI Xue-fan“; HUANG Chang-xing; SUN Yong—tao; PAN Lei; LI Guang—yu; WANG Lin—XH

PDF
Cite
Share

facebook

twitter

google

linkedin

February 2003

ZHANG Ying, BAI Xue-fan“, HUANG Chang-xing, SUN Yong—tao, PAN Lei, LI Guang—yu and WANG Lin—XH. HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection[J]. Virologica Sinica, 2003, 18(1): 23-26.

Citation: ZHANG Ying, BAI Xue-fan“, HUANG Chang-xing, SUN Yong—tao, PAN Lei, LI Guang—yu, WANG Lin—XH. HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection .VIROLOGICA SINICA, 2003, 18(1) : 23-26.

HIV-1辅受体配体的融合表达载体的构建及表达

张颖白雪帆黄长形孙永涛潘蕾李光玉王临旭 ,

1.
第四军医大学唐都医院全军感染病诊疗中心陕西西安

摘要

应用PCR扩增RANTES-KDEL基因,鉴定后与真核表达载体pCMV—S/K连接,构建成HIV-1辅受体的配体、趋化因子RANTES和SDF-1的融合表达载体pCMV-R—K-S—K,酶切鉴定和测序证明成功构建了pCMV—R—K—S—K融合表达载体。脂质体介导pCMV—R—K-S—K转染HeLa细胞,间接免疫荧光证实RANTES和SDF-1可高效表达于HeLa细胞。结果表明构建的pCMV—R—K—S—K融合表达载体能在HeLa细胞中高效表达,可用于下一步的HIV-1感染实验。
- HIV-1
- , 辅受体
- , 趋化因子
- , 融合表达载体
- , 转染

HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection

1.
The Center of Diagnosis and Treotment for Infectious Diseases ofPLA，Tangdu Hospital，FMMU，Xi'an710038，China

Abstract

In order to construct bicistronic expression vector of RANTES and SDF-l,the ligands of HIV-1 principal coreceptors,RANTES-KDEL were amplified from pCMV-R-K by PCR and ligased with eukaryotic expression vector pCMV-S/K. The construction of pCMV-R-K-S-K was confirmed by enzymatic digestion and sequencing. Then pCMV-R-K-S-K transfection into HeLa cells was carried out by lipofectin. RAN TES and SDF-1 were showed expressed in HeLa cells by indirect immumofluo-rescence. The results show that pCMV-R-K-S-K was constructed and expressed in cell line HeLa successfully, which will contribute to gene therapy of HIV-1.

References
Proportional views

PDF

Article Metrics

Article views(4688) PDF downloads(873) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection

1. The Center of Diagnosis and Treotment for Infectious Diseases ofPLA，Tangdu Hospital，FMMU，Xi'an710038，China

Abstract: In order to construct bicistronic expression vector of RANTES and SDF-l,the ligands of HIV-1 principal coreceptors,RANTES-KDEL were amplified from pCMV-R-K by PCR and ligased with eukaryotic expression vector pCMV-S/K. The construction of pCMV-R-K-S-K was confirmed by enzymatic digestion and sequencing. Then pCMV-R-K-S-K transfection into HeLa cells was carried out by lipofectin. RAN TES and SDF-1 were showed expressed in HeLa cells by indirect immumofluo-rescence. The results show that pCMV-R-K-S-K was constructed and expressed in cell line HeLa successfully, which will contribute to gene therapy of HIV-1.