Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

YU Hong-chun; ZHENG Gui-ling; WANG Xiao-yun; LI Guo—xun; ZHAO Kui-jun

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February 2003

YU Hong-chun, ZHENG Gui-ling, WANG Xiao-yun, LI Guo—xun and ZHAO Kui-jun. Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV[J]. Virologica Sinica, 2003, 18(1): 31-34.

Citation: YU Hong-chun, ZHENG Gui-ling, WANG Xiao-yun, LI Guo—xun, ZHAO Kui-jun. Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV .VIROLOGICA SINICA, 2003, 18(1) : 31-34.

粘虫胚胎细胞系的建立及对MsNPV敏感性的测定

1.
1．东北农业大学农学院植保系，黑龙江哈尔滨150030
2.
河北农业大学，河北保定071001

摘要

用培养24h的粘虫受精卵成功地建立一株粘虫胚胎细胞后，命名为NEAU—Ms一980312，目前已传代201 代．该细胞系主要含有圆形和纺锤形两种细胞，在28℃ 、l20h的细胞群体倍增时间为47．82h。该细胞系对粘虫核型多角体病毒有较高的敏感性，用原代病毒以感染复数(m．O．i．)为0．08TCID ，细胞感染细胞，在接毒后lOd，病毒感染率和每细胞产生的多角体数量分别是为62．10％和33．59PIB。
- 粘虫
- , 细胞系
- , 粘虫核型多角体病毒(MsNPV)
- , 敏感性

Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

1.
1．Northeast Agricultural University,Harbin 150030，China．,2．Hebei Agricultural University,Baoding 071001，China

Abstract

Abstract： A new cell line，designated NEAU—Ms一9803 12，was established from 24一hr—old Mythimna separata eggs and was passaged 201 times．The cells were heterogeneous in morphology with spherical and spindle—shaped cells．Th e population doubling time of the cell line aws 47．82h in 120h at 28~C． The cell line was highly susceptible to Mythimna separata nll~leal"polyhedrosis vu’us (MsNPV)．When the cells were inoculated at a multiplicity of infection (m．o．i．)of 0．08TCID50／cell with wild pas— sage MsNPV。the infection rates and occlusion bodies f OB) yield reached a value of 62．10％， 33．59PIB／infected cell at 240h postinfection(pi)，respectively
- Mythimna separata
- , Cell line
- , Mythimna separata nll~leol"polyhedrosis virus(MsNPV)
- , SUS ceptibilit

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

1. 1．Northeast Agricultural University,Harbin 150030，China．,2．Hebei Agricultural University,Baoding 071001，China

Abstract: Abstract： A new cell line，designated NEAU—Ms一9803 12，was established from 24一hr—old Mythimna separata eggs and was passaged 201 times．The cells were heterogeneous in morphology with spherical and spindle—shaped cells．Th e population doubling time of the cell line aws 47．82h in 120h at 28~C． The cell line was highly susceptible to Mythimna separata nll~leal"polyhedrosis vu’us (MsNPV)．When the cells were inoculated at a multiplicity of infection (m．o．i．)of 0．08TCID50／cell with wild pas— sage MsNPV。the infection rates and occlusion bodies f OB) yield reached a value of 62．10％， 33．59PIB／infected cell at 240h postinfection(pi)，respectively