Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species

WANG Yong-shan; Zhou Zong-an; DENG Xiao-zhao; ZHAO Xin-tai; HOU Ji-bo; HE Kong-wang; DING Chan; LIU Yu; GAO Jian; DIAO Zhen-yu

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February 2003

WANG Yong-shan, Zhou Zong-an, DENG Xiao-zhao, ZHAO Xin-tai, HOU Ji-bo, HE Kong-wang, DING Chan, LIU Yu, GAO Jian and DIAO Zhen-yu. Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species[J]. Virologica Sinica, 2003, 18(1): 68-71.

Citation: WANG Yong-shan, Zhou Zong-an, DENG Xiao-zhao, ZHAO Xin-tai, HOU Ji-bo, HE Kong-wang, DING Chan, LIU Yu, GAO Jian, DIAO Zhen-yu. Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species .VIROLOGICA SINICA, 2003, 18(1) : 68-71.

传染性法氏囊病病毒不同分离株vp2基因部分核酸序列分析

1.
1．南京军区军事医学研究所，江苏南京210001
2.
上海市肿瘤研究所癌基因与相关基因国家重点实验室，上海200032
3.
江苏省农业科学院兽医研究所，江苏南京210014
4.
江苏省家禽科学研究所，江苏扬州225003

摘要

根据已报告的传染性法氏囊病病毒(Infectious bursal disease virus，IBDV)cDNA序列，设计引物，用R r_PCR扩增CH (鸡)、DU (鸭)、GE (鹅)和SP (麻雀)四种不同源IBDV分离株的vp2基因高变区。核酸序列测定分析表明，四种不同源IBDV分离株vp2基因高变区的同源性为97％．推导编码蛋白氨基酸序列的同源性98％，两个亲水区和七肽区的氨基酸序列完全一致。本研究结果提示，自然感染IBDV 的鸭、鹅和麻雀不仅可成为病毒携带者或传染源，而且在病毒变异中起一定作用。
- 麻雀
- , 鸭
- , 鹅
- , 传染性法氏囊病病毒：VP2
- , 序列分析

Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species

1.
1．HuadongResearchInstitute ofMedicalBiotechnics，Nanjing210002，China
2.
NationalLaboratoryforOncogenes andRelatedGenes，．Shanghai Cancer Institute，Shanghai 200032，China
3.
Institute ofVeterinaryScience，JiangsuAcademyofAgricultural Sciences，Nanjing210014，China
4.
Jiangsu Institute ofPoultry Science，Yangzhou 225003，China

Abstract

Abstract：According to the published cDNA sequences of Infectious bursal disease virus(IBDV)，a pairof primers to variable region in VP2 were designed for cDNA synthesis and PCR amplification of fourIBDV isolated from different bird species，CH，DU，GE and SP．The nucleotide sequences determinedhave been compared to each other．Th e four isolates are closely related，the homology of the four iso—lates being 97％ at the nucleotied level and 98％ at the amino acid leve1．Th eir two hydrophilic regionsand one heptapeptide were identical at the amino acid leve1． Th e results revealed that the ducks，geeseand sparrows naturally infected with IBDV not only could become the carriers or reservoirs in transmis—sion but may take certain effect on the variation of IBDV．
- Sparow
- , Duck
- , Goose
- , Infectious bursal disease virus
- , VP2

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species

1. 1．HuadongResearchInstitute ofMedicalBiotechnics，Nanjing210002，China
2. NationalLaboratoryforOncogenes andRelatedGenes，．Shanghai Cancer Institute，Shanghai 200032，China
3. Institute ofVeterinaryScience，JiangsuAcademyofAgricultural Sciences，Nanjing210014，China
4. Jiangsu Institute ofPoultry Science，Yangzhou 225003，China

Abstract: Abstract：According to the published cDNA sequences of Infectious bursal disease virus(IBDV)，a pairof primers to variable region in VP2 were designed for cDNA synthesis and PCR amplification of fourIBDV isolated from different bird species，CH，DU，GE and SP．The nucleotide sequences determinedhave been compared to each other．Th e four isolates are closely related，the homology of the four iso—lates being 97％ at the nucleotied level and 98％ at the amino acid leve1．Th eir two hydrophilic regionsand one heptapeptide were identical at the amino acid leve1． Th e results revealed that the ducks，geeseand sparrows naturally infected with IBDV not only could become the carriers or reservoirs in transmis—sion but may take certain effect on the variation of IBDV．