Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate W X84

YAN Weiweil; CUI Zizhong; WANG Yongkun

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April 2003

YAN Weiweil, CUI Zizhong and WANG Yongkun. Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate W X84[J]. Virologica Sinica, 2003, 18(2): 129-133.

Citation: YAN Weiweil, CUI Zizhong, WANG Yongkun. Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate W X84 .VIROLOGICA SINICA, 2003, 18(2) : 129-133.

兔出血症病毒中国株衣壳蛋白基因的克隆和序列分析

1.
1．扬州大学畜牧兽医学院动物医学系，江苏扬州，225009
2.
山东农业大学动物科技系，山东泰安，271018

摘要

摘要：应用RT-PCR技术，从兔出血症病毒中国分离株WX84中成功扩增出预期大小为1．7kb的特异性条带，将扩增产物提纯后克隆入pGEM -T载体，经转化、筛选及酶切鉴定后，获得了该株病毒衣壳蛋白基因的克隆，序列分析表明扩增的中国株RHDV衣壳蛋白基因片段长度为1 740bp，共编码580个氨基酸。该核酸序列与其它国家报道的多株RHDV序列相互间同源性高达98．2％~99．0％，其推导的氨基酸序列同源性也达98．3％~99．1％，为极度保守片段。
- 兔出血症病毒(RHDV)
- , 衣壳蛋白基因(vp60)
- , 序列分析：反转录聚合酶链式反应(RT-PCR)

Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate W X84

1.
Animal Science and Veterinary Medicine College Yangzhou University , Yangzhou，225009，China
2.
Animal Science College Shandong Agricultural University,Taian，271018，China

Abstract

Abstract：The capsid protein(VP60)gene of Rabbit hemorrhagic disease virus(RHDV)isolate WX84 was amplified by RT．PCR and cloned into pGEM R-T vector for sequence．Th e sequence an alysis showed that the nucleotide sequence of the vp60 gene of WX84 was 1 740 bp an d encoding a protein of 580 amino acids．Sequence comparison with other published RHDV vp60 genes showed that the homology of the nucleotide was between 98．2％ and 99．0％．Th e result showed that vp60 gene was a highly conserved gene．
- Rabbit hemorrhagic disease virus
- , Capsid protein gene(vp60)
- , RT—PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate W X84

1. Animal Science and Veterinary Medicine College Yangzhou University , Yangzhou，225009，China
2. Animal Science College Shandong Agricultural University,Taian，271018，China

Abstract: Abstract：The capsid protein(VP60)gene of Rabbit hemorrhagic disease virus(RHDV)isolate WX84 was amplified by RT．PCR and cloned into pGEM R-T vector for sequence．Th e sequence an alysis showed that the nucleotide sequence of the vp60 gene of WX84 was 1 740 bp an d encoding a protein of 580 amino acids．Sequence comparison with other published RHDV vp60 genes showed that the homology of the nucleotide was between 98．2％ and 99．0％．Th e result showed that vp60 gene was a highly conserved gene．