Cloning and Sequencing off gene of Avian paramyxovirus Type I Isolate

SONG Jian—ling; ZHAO Wen—hua; YANG Bin; ZHANG Yong—xian; WANG Jin—ping; XIN Ai—guo; ZHU Jian—bo; CONG Pei—qing

PDF
Cite
Share

facebook

twitter

google

linkedin

April 2003

SONG Jian—ling, ZHAO Wen—hua, YANG Bin, ZHANG Yong—xian, WANG Jin—ping, XIN Ai—guo, ZHU Jian—bo and CONG Pei—qing. Cloning and Sequencing off gene of Avian paramyxovirus Type I Isolate[J]. Virologica Sinica, 2003, 18(2): 141-145.

Citation: SONG Jian—ling, ZHAO Wen—hua, YANG Bin, ZHANG Yong—xian, WANG Jin—ping, XIN Ai—guo, ZHU Jian—bo, CONG Pei—qing. Cloning and Sequencing off gene of Avian paramyxovirus Type I Isolate .VIROLOGICA SINICA, 2003, 18(2) : 141-145.

禽I型副粘病毒厂基因克隆及序列分析

1.
农业部热带亚热带动物病毒学重点开放实验室，云南昆明，650224

摘要

用RT—PCR一步法对云南省不同禽类(鸡、鸽子)3株禽I型副粘病毒F基因进行扩增和克隆，并对其厂基因片段核苷酸序列进行分析，结果表明，云南省禽I型副粘病毒各毒株同源性为88．1％～94．9％，与疫苗株LaSom 和强毒株F48E9的同源性为85．6％。所分离两株新城疫病毒在F蛋白裂解位点区(112～117aa)的氨基酸序列与强毒株在这一区域的序列完全相同，表明为强毒株。鸽I型副粘病毒F蛋白裂解位点区的氨基酸序列与PPMVZQ98—1 株在这一区域的序列完全相同，揭示为中强毒株。以1 662bp核苷酸绘制系统发育树，表明云南地方新城疫病毒属于基因Ⅶ型，鸽I型副粘病毒属于基因Ⅵ型。
- 禽I型副粘病毒
- , f基因
- , 克隆
- , 序列分析
- , F蛋白

Cloning and Sequencing off gene of Avian paramyxovirus Type I Isolate

1.
The Key Openning Laboratory of Tropical and Subtropical AnimalVirology of Ministry of Agriculture，Kunming 650224， China

Abstract

Abstract：The f gene was amplified with one—step RT—PCR，cloned and sequenced from three virus isolates(Avian paramyxovirus type I)，which were isolated from diferent avian (chicken and pigeon) inYunnan province．Th e result of the nucleotide sequence an alysis indicated that the homology between the three strains studied was 88．1％-94．9％：while the studied strains with refe；rence strains LaSota and F48F-,9 only showed 85．6％ homology．Th e study on the cleavage site amino acid sequence deduced from the nucleotide sequence(residues 1 1 2-1 1 7)showed that the two NDV su~ns matched to all NDV velogenic strains and the Pigeon paramyxovirus(PPMV)I strain matched to PPMV ZQ98—1 strain(one mesogenic strain)，SO the former were velogenic strains and the later was mesogenic strain．Phylogenetic tree constructed with 16 reference strains of 1 662nt(ORF region)of thef gene showed that the two NDV strains should belongs to genotype VII an d the PPM V strain belongs to genotype VI．
- Avian paramyxovirus type I
- , f gene
- , Clone
- , Sequence analysis

References
Proportional views

PDF

Article Metrics

Article views(3836) PDF downloads(1013) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequencing off gene of Avian paramyxovirus Type I Isolate

1. The Key Openning Laboratory of Tropical and Subtropical AnimalVirology of Ministry of Agriculture，Kunming 650224， China

Abstract: Abstract：The f gene was amplified with one—step RT—PCR，cloned and sequenced from three virus isolates(Avian paramyxovirus type I)，which were isolated from diferent avian (chicken and pigeon) inYunnan province．Th e result of the nucleotide sequence an alysis indicated that the homology between the three strains studied was 88．1％-94．9％：while the studied strains with refe；rence strains LaSota and F48F-,9 only showed 85．6％ homology．Th e study on the cleavage site amino acid sequence deduced from the nucleotide sequence(residues 1 1 2-1 1 7)showed that the two NDV su~ns matched to all NDV velogenic strains and the Pigeon paramyxovirus(PPMV)I strain matched to PPMV ZQ98—1 strain(one mesogenic strain)，SO the former were velogenic strains and the later was mesogenic strain．Phylogenetic tree constructed with 16 reference strains of 1 662nt(ORF region)of thef gene showed that the two NDV strains should belongs to genotype VII an d the PPM V strain belongs to genotype VI．