Inclusion Body--An Important Morphological Marker of Infectious canine hepatitis virus

CHANG Guo—quan; FENG Shu—zhang

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April 2003

CHANG Guo—quan and FENG Shu—zhang. Inclusion Body--An Important Morphological Marker of Infectious canine hepatitis virus[J]. Virologica Sinica, 2003, 18(2): 152-154.

Citation: CHANG Guo—quan, FENG Shu—zhang. Inclusion Body--An Important Morphological Marker of Infectious canine hepatitis virus .VIROLOGICA SINICA, 2003, 18(2) : 152-154.

包涵体一犬传染性肝炎病毒形态发生学的重要特征

常国权 ,
冯书章

1.
解放军军需大学军事兽医研究所，吉林长春，130062

摘要

用免疫金电镜技术和电镜原位杂交技术观察了犬传染性肝炎病毒感染的犬肾传代细胞中的病毒包涵体，发现这些包涵体具有以下三种基本形态：1．松散均质包涵体；2．副结晶色包涵体；3．致密颗粒包涵体。其中前二种包涵体能被免疫金标记，它们是尚未成病毒粒子的病毒蛋白或是病毒装配后乘余的病毒蛋白，后一种包涵体能被病毒DNA探针标记，是病毒核酸合成过剩而堆积起来形成的产物。此外，本文还描述和讨论了包涵体与细胞及病毒发育成熟的关系。
- 犬传染性肝炎病毒
- , 包涵体
- , 形态发生学
- , 免疫金电镜技术
- , 电镜原位杂交技术

Inclusion Body--An Important Morphological Marker of Infectious canine hepatitis virus

1.
The Military Veterinary Institute．Quartermaster University ofPLA，Changchun 130062，China

Abstract

Morphological characterization of Infectious canine hepatitis virus specific inclusion bodies was carried out by both immunogold electron microscopy and electron microscopy in sire hybridization． Three morphological types of inclusion bodies were observed：loose homogeneous，paracrystalline an d dense gran ular inclusion body．The former two labelled with immunogold are viral an tigenic protein of viral particles，the latter one labelled with viral DNA probe is composed of viral DNA．Th e possible relationship among the inclusion bodies an d their be aring to virus morphogenesis were discussed
- Infectious canine hepatitis virus
- , Inclusion body
- , Morphogenesis
- , Immunogold electron microscopy
- , Electron microscopy in situ hybridization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Inclusion Body--An Important Morphological Marker of Infectious canine hepatitis virus

1. The Military Veterinary Institute．Quartermaster University ofPLA，Changchun 130062，China

Abstract: Morphological characterization of Infectious canine hepatitis virus specific inclusion bodies was carried out by both immunogold electron microscopy and electron microscopy in sire hybridization． Three morphological types of inclusion bodies were observed：loose homogeneous，paracrystalline an d dense gran ular inclusion body．The former two labelled with immunogold are viral an tigenic protein of viral particles，the latter one labelled with viral DNA probe is composed of viral DNA．Th e possible relationship among the inclusion bodies an d their be aring to virus morphogenesis were discussed