Construction and Expression of Periplanete fuliginosa densovirus Recombinant Plasmid W hich Contains Luciferase Gene

YANG Juan; ZHANG Jia—min; JIANG Hong; DENG Xiao-jun; HU Jian—fang; HU Yuang—yang*

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April 2003

YANG Juan, ZHANG Jia—min, JIANG Hong, DENG Xiao-jun, HU Jian—fang and HU Yuang—yang*. Construction and Expression of Periplanete fuliginosa densovirus Recombinant Plasmid W hich Contains Luciferase Gene[J]. Virologica Sinica, 2003, 18(2): 178-180.

Citation: YANG Juan, ZHANG Jia—min, JIANG Hong, DENG Xiao-jun, HU Jian—fang, HU Yuang—yang*. Construction and Expression of Periplanete fuliginosa densovirus Recombinant Plasmid W hich Contains Luciferase Gene .VIROLOGICA SINICA, 2003, 18(2) : 178-180.

含荧光素酶基因的黑胸大蠊浓核病毒重组质粒的构建与表达

1.
武汉大学病毒研究所，湖北武汉，430072

摘要

Abstract：A luciferase gene has been inserted into the recombinan t plasmid PIDNV—pUCl l9 which contained partly deletion of genome of Periplanete fuliginosa densovirus(PfDNV)．The recombinant plasmid with luciferase gene was co··transfected with pfDNV·pUCl l9 into Periplanelefuliginosa larvae an d had a high luciferase gene expression in enteron of the tran sfected larvae．
- 黑胸大蠊
- , 浓核病毒
- , 重组质粒
- , 荧光素酶

Construction and Expression of Periplanete fuliginosa densovirus Recombinant Plasmid W hich Contains Luciferase Gene

1.
Institute ofVirology,Wuhan University,Wuhan 430072，China

Abstract

Abstract：A luciferase gene has been inserted into the recombinan t plasmid PIDNV—pUCl l9 which contained partly deletion of genome of Periplanete fuliginosa densovirus(PfDNV)．The recombinant plasmid with luciferase gene was co··transfected with pfDNV·pUCl l9 into Periplanelefuliginosa larvae an d had a high luciferase gene expression in enteron of the tran sfected larvae．
- Periplanetefuliginosa
- , Densovirus
- , Recombinant Plasmid
- , Luciferase

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Expression of Periplanete fuliginosa densovirus Recombinant Plasmid W hich Contains Luciferase Gene

1. Institute ofVirology,Wuhan University,Wuhan 430072，China

Abstract: Abstract：A luciferase gene has been inserted into the recombinan t plasmid PIDNV—pUCl l9 which contained partly deletion of genome of Periplanete fuliginosa densovirus(PfDNV)．The recombinant plasmid with luciferase gene was co··transfected with pfDNV·pUCl l9 into Periplanelefuliginosa larvae an d had a high luciferase gene expression in enteron of the tran sfected larvae．