Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus

LU Yin-hua; XU Lihua; HUA Xiu-guo; TAN Guo; HUANG Wei-jian; CHEN Desheng; CHEN Puyan

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April 2003

LU Yin-hua, XU Lihua, HUA Xiu-guo, TAN Guo, HUANG Wei-jian, CHEN Desheng and CHEN Puyan. Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus[J]. Virologica Sinica, 2003, 18(2): 184-186.

Citation: LU Yin-hua, XU Lihua, HUA Xiu-guo, TAN Guo, HUANG Wei-jian, CHEN Desheng, CHEN Puyan. Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus .VIROLOGICA SINICA, 2003, 18(2) : 184-186.

猪圆环病毒2型及猪繁殖与呼吸综合症病毒的快速检测

芦银华 ¹ ,
许立华 ,
华修国 ¹ ,
谈国蕾 ¹ ,
黄伟坚 ¹ ,
陈德胜 ¹ ,
陈溥言 ^1*

1.
1．南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京，210095
2.
宁夏大学农学院动物科学系，宁夏银川，750105
3.
上海交通大学农学院，上海，200030

摘要

Abstract：According to the published genome seqflences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV)，primers were designed and PCR， RT-PCR were set up for the detection of PCV2 an d PRRS respectively．W ith the established meth ods， 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 an d PRRSV．The results demonstrated that 22 sam ples were posirive for PCV2，27 sam ples were positive for PRRSV an d am ong th e above positive sam ples，1 8 sam ples were positive fo r both viruses．The data obtained in the present study indicated that PCV2 an d PRRSV maybe play an important role in the course of the development of respiratory diseases．
- Key words： Porcine circovirus type 2：Porcine reproductive and respiratory synd rome virus
- , COinfection
- , Detection

Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus

1.
1．Key laboratory of animal disease diagnostic and immunology,Ministry of Agriculture Nanjing agricultural university, Nanjing 210095，China
2.
Animal Science Department of Agricultural college，Ningxia University, Hnchuan，750105，China-3．College ofagriculture,Shanghai Jiaotong University,Shanghai，200030，China

Abstract

Abstract：According to the published genome seqflences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV)，primers were designed and PCR， RT-PCR were set up for the detection of PCV2 an d PRRS respectively．W ith the established meth ods， 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 an d PRRSV．The results demonstrated that 22 sam ples were posirive for PCV2，27 sam ples were positive for PRRSV an d am ong th e above positive sam ples，1 8 sam ples were positive fo r both viruses．The data obtained in the present study indicated that PCV2 an d PRRSV maybe play an important role in the course of the development of respiratory diseases．
- Porcine circovirus type 2：Porcine reproductive and respiratory synd rome virus
- , CO．infection
- , Detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus

1. 1．Key laboratory of animal disease diagnostic and immunology,Ministry of Agriculture Nanjing agricultural university, Nanjing 210095，China
2. Animal Science Department of Agricultural college，Ningxia University, Hnchuan，750105，China-3．College ofagriculture,Shanghai Jiaotong University,Shanghai，200030，China

Abstract: Abstract：According to the published genome seqflences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV)，primers were designed and PCR， RT-PCR were set up for the detection of PCV2 an d PRRS respectively．W ith the established meth ods， 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 an d PRRSV．The results demonstrated that 22 sam ples were posirive for PCV2，27 sam ples were positive for PRRSV an d am ong th e above positive sam ples，1 8 sam ples were positive fo r both viruses．The data obtained in the present study indicated that PCV2 an d PRRSV maybe play an important role in the course of the development of respiratory diseases．