摘要：应用PCR对121例宫颈脱落细胞和组织分别检测HPV。16／18和HSV一2 DNA。同时应用免疫组化S—P法检 测76例宫颈组织中p53过度表达。结果发现，宫颈癌组织中HPV．16／18和HSV-2阳性率分别为61．3％和32．3％， 慢性宫颈炎组HPV一16／18和HSV一2阳性率分别为22．5％和20．0％，与正常宫颈组比较均有显著性差异。宫颈癌中 HPV一16／18和HSV一2混合感染率为16．1％。p53过度表达率在慢性宫颈炎、宫颈上皮内瘤变(CIN)、宫颈癌组织 中呈梯度递增。另外，宫颈癌组织中 3过度表达与HPV一16／18、HSV一2的感染无相关性。提示：宫颈癌的发生 与HPV一16／18关系密切，HSV一2可能与HPV一16／18协同作用导致宫颈癌的发生。宫颈组织中 3过度表达率与宫 颈癌的进程有关，这在宫颈癌的防治方面有一定的临床意义。

Abstract： Wle examined 121 Specimens of exfoliated cells and tissue of cervix for the presence of肿V一16／18 an d HSV一2 DNA by PCR．At the same time，we analysised 76 specimens from tissue ofcervix for the expression of p53 by immunohistochemical assay．The HPV一16／18 was found in 19(61．3％)of 31 cervical carcinoma，in 9(22．5％)of 40 chronic cervicitis，in 3(6．O％)of 50 normaluterine cervix．The positive rate of HSV一2 was 32．3％ (10／31)，2O．O％ (8，4O)and 4．0％ (2／50)．1 emixed infection rate of肿V 16／18 an d HSV一2 was 16．1％ in cervical carcinoma．The rates of positiveexpression ofp53 was found to have a gradient of crescendo in the tissues of chronic cervicitis．CIN an dcervical carcinoma．Wle found that cerv ical carcinoma is strongly related witll HPV一16／18 infection．Asynergetic action may OCCUr between HPv an d HSV一2 infections in the oncogenesis of cervicalcarcinoma．Th e overepression of p53 appears to be closely associated with the development of cervicalcan cer．Th ere is a matter ofclinical significan ce on the prevention an d treatment ofcervical carcinoma．