cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

ZHAO Shu—ling; LIANG Chang—yong; HONG Jing-jun; PENG Hui—yin*

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June 2003

ZHAO Shu—ling, LIANG Chang—yong, HONG Jing-jun and PENG Hui—yin*. cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus[J]. Virologica Sinica, 2003, 18(3): 246-250.

Citation: ZHAO Shu—ling, LIANG Chang—yong, HONG Jing-jun, PENG Hui—yin*. cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus .VIROLOGICA SINICA, 2003, 18(3) : 246-250.

马尾松毛虫质型多角体病毒S4的cDNA克隆及序列分析

1.
中国科学院武汉病毒研究所，湖北武汉，430071

摘要

从马尾松毛虫质型多角体病毒湖南株中提取病毒核酸，回收、纯化第四片段S4，经RT-PCR 扩增得到了 S4的cDNA克隆并测定了其全序列。结果表明，S4全长由3 262个碱基组成，包含一个编码1058个氨基酸的完整开放阅读框架。Blast同源分析显示，DpCPV S4与LdCPV S4、BmCPV S4和RRSV S2编码蛋白氨基酸的同源性分别为94％、92％和22％。另外，氨基酸序列部分区域与Methanosarcina mazei Goel的甲基化转移酶有同源性，且序列中含有鸟苷酸转移酶活性位点。
- 马尾松毛虫质型多角体病毒
- , S4
- , 多聚腺苷化
- , 序列分析

cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

1.
Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan430071，China

Abstract

Abstract：Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag extracted an d S4 was separated．RT—PCR amplification technique Wag adopted to clone the viral genomic dsRNA S4 flor sequence determination．The results showed：the full length of S4 is 3262bp，it contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length．Tlle similarity of predicted protein were 94％、92％ an d 22％ with LdCPV、BmCPV an d RRSV respectively． Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs were also present in the deduced protein．
- Sequence an alysis
- , Dendrolimus punctatus cytoplasmic polyhedrosis virus
- , S4：Polyadenyl—ation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

1. Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan430071，China

Abstract: Abstract：Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag extracted an d S4 was separated．RT—PCR amplification technique Wag adopted to clone the viral genomic dsRNA S4 flor sequence determination．The results showed：the full length of S4 is 3262bp，it contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length．Tlle similarity of predicted protein were 94％、92％ an d 22％ with LdCPV、BmCPV an d RRSV respectively． Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs were also present in the deduced protein．