LIU De, DUAN Yuan and Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene[J]. Virologica Sinica, 2003, 18(3): 254-258.
Citation:
LIU De, DUAN Yuan, .
Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene .VIROLOGICA SINICA, 2003, 18(3)
: 254-258.
双拷贝v-cath基因的重组HaNPV的构建及毒力分析
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摘要
摘要:利用HaNPV的Bac—to—Bac系~(Hanpvid)构建了双拷贝v—cath基因的重组HaNPV,即:除病毒自身的v—cath 基因外,还带有由iel启动子控制的早期表达v.cath基因的重组病毒dciHaNPV。Dotblot和No~ emblot分析证 明:重组病毒构建正确。用重组病毒感染Hz—AM1细胞,测定了dciHaNPV的TCID50为3.16~10 TCIDso/mL。
Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene
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Abstract
Abstract:A recombinant virus containing double copies of V—cath gene was constructed by using Han pvid Bac—to—Bac system.ThiS recombinan t virus was named as dciHaNP、 because it was inserted an other copy of V-cath gene controlled by iel promoter.Dot blot an d Northern blot an alysis showed that this recombinan t virus was correct.The toxicity was measured by using Hz—AM l cells infec ted with the recombinan t virus.Th e TCID5n ofdciHaNPlV is 3.16× l0 TCID5o/mL.
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References
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Proportional views
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