Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene

LIU De; DUAN Yuan

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June 2003

LIU De, DUAN Yuan and Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene[J]. Virologica Sinica, 2003, 18(3): 254-258.

Citation: LIU De, DUAN Yuan, . Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene .VIROLOGICA SINICA, 2003, 18(3) : 254-258.

双拷贝v-cath基因的重组HaNPV的构建及毒力分析

1.
1．华中师范大学生命科学学院，武汉，430079：2．武汉大学病毒研究所，武汉，430072

摘要

摘要：利用HaNPV的Bac—to—Bac系~(Hanpvid)构建了双拷贝v—cath基因的重组HaNPV，即：除病毒自身的v—cath 基因外，还带有由iel启动子控制的早期表达v．cath基因的重组病毒dciHaNPV。Dotblot和No~ emblot分析证明：重组病毒构建正确。用重组病毒感染Hz—AM1细胞，测定了dciHaNPV的TCID50为3．16~10 TCIDso／mL。
- 组织蛋白酶基因v-cath
- , Northern blot分析
- , Bac—to—Bac系统
- , 重组杆状病毒

Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene

LIU De ,
DUAN Yuan ,
²

1.
College of Life Science,Central China Normal University,Wuhan 430079,China

Abstract

Abstract：A recombinant virus containing double copies of V—cath gene was constructed by using Han pvid Bac—to—Bac system．ThiS recombinan t virus was named as dciHaNP、 because it was inserted an other copy of V-cath gene controlled by iel promoter．Dot blot an d Northern blot an alysis showed that this recombinan t virus was correct．The toxicity was measured by using Hz—AM l cells infec ted with the recombinan t virus．Th e TCID5n ofdciHaNPlV is 3．16× l0 TCID5o／mL．
- Cathepsin gene V-cath
- , Northern blot an alysis
- , Bac—to—Bac system
- , Recombinant baculovirus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene

1. College of Life Science,Central China Normal University,Wuhan 430079,China

Abstract: Abstract：A recombinant virus containing double copies of V—cath gene was constructed by using Han pvid Bac—to—Bac system．ThiS recombinan t virus was named as dciHaNP、 because it was inserted an other copy of V-cath gene controlled by iel promoter．Dot blot an d Northern blot an alysis showed that this recombinan t virus was correct．The toxicity was measured by using Hz—AM l cells infec ted with the recombinan t virus．Th e TCID5n ofdciHaNPlV is 3．16× l0 TCID5o／mL．