W U, LIU Yu, Zhang Heng, GU Chao-jiang, ZHENG Cong, Qu San, WANG Cun and CHANG Hui. 口蹄疫病毒感染BHK-21细胞的代谢热谱研究[J]. Virologica Sinica, 2003, 18(3): 283-287.
Citation: W U, LIU Yu, Zhang Heng, GU Chao-jiang, ZHENG Cong, Qu San, WANG Cun, CHANG Hui. 口蹄疫病毒感染BHK-21细胞的代谢热谱研究 .VIROLOGICA SINICA, 2003, 18(3) : 283-287.

口蹄疫病毒感染BHK-21细胞的代谢热谱研究

  • 本文应用高灵敏度的LKB2277生物活性检测仪测定FMDV感染BHK-21细胞的代谢热谱,并与传统方法测定的一步生长曲线进行比较,二者具有显著的相似性。结果表明,微量热法通过对细胞及其受病毒感染的细胞体系代谢热的测定,能有效地监测病毒在宿主细胞内增殖的过程。该方法还提供了一种动态连续分析病毒感染增殖的新手段。

口蹄疫病毒感染BHK-21细胞的代谢热谱研究

  • Both microcalorimetric measurement analysis by using LKB2277 bioactivity monitor and traditional method used in one-step growth curve in virology were carded out on BHK-21 cell line infected by FMDV (Foot-and-Mouth Disease Virus). The results show that microcalorimetric study can be applied in virus infection research through measuring metabolic energy released from BHK-21 cell and BHK-21 cell infected by FMDV. Meantime this microcalorimetric method provided a kind of new dynamic continuous analysis method on the virus infection research.

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    口蹄疫病毒感染BHK-21细胞的代谢热谱研究

    • 1. College of Life Science,Wuhan University,Wuhan 430072,China

    Abstract: Both microcalorimetric measurement analysis by using LKB2277 bioactivity monitor and traditional method used in one-step growth curve in virology were carded out on BHK-21 cell line infected by FMDV (Foot-and-Mouth Disease Virus). The results show that microcalorimetric study can be applied in virus infection research through measuring metabolic energy released from BHK-21 cell and BHK-21 cell infected by FMDV. Meantime this microcalorimetric method provided a kind of new dynamic continuous analysis method on the virus infection research.

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