Verifying the Specific Binding Between W SSV and Shrimp Gill Cell Membrane

YI Zhi-gang; HUANG Jie; XIE Fei-xia; JIA Peiqiao; LI Yun

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June 2003

YI Zhi-gang, HUANG Jie, XIE Fei-xia, JIA Peiqiao and LI Yun. Verifying the Specific Binding Between W SSV and Shrimp Gill Cell Membrane[J]. Virologica Sinica, 2003, 18(3): 295-297.

Citation: YI Zhi-gang, HUANG Jie, XIE Fei-xia, JIA Peiqiao, LI Yun. Verifying the Specific Binding Between W SSV and Shrimp Gill Cell Membrane .VIROLOGICA SINICA, 2003, 18(3) : 295-297.

对虾白斑综合症病毒与虾鳃细胞膜特异性结合关系的确立

1.
1．中国水产科学研究院黄海水产研究所，青岛，266071
2.
中国海洋大学海洋生命学院，青岛，266003

摘要

Abstract：Cell membran e extracted from shrimp gill was coated on 96 well plate and bound witIl DIG labeled White spot syndrome virus(wssv)，then detected with Anti—DIG—Fab—HRP system，meanwhile taking the cell membran e from hepatopancreas，muscle，and E．coli for control group，the result verified the specific binding between W SSV an d gill cell membran e ．And here the binding assay can be employed as a convenient an d rapid way to evaluate the binding nature between W SSV an d the shrimp gill cell membran e．
- 对虾白斑综合症病毒(white spot syndrome virus,WSSV)
- , 细胞膜
- , 结合分析

Verifying the Specific Binding Between W SSV and Shrimp Gill Cell Membrane

1.
Yellow Sea Fisheries Research Institute，Chinese Academy ofFishery Sciences,Qingdao 266071，China
2.
Ocean UniversityofChina Qingdao 266003，China

Abstract

Abstract：Cell membran e extracted from shrimp gill was coated on 96 well plate and bound witIl DIG labeled White spot syndrome virus(wssv)，then detected with Anti—DIG—Fab—HRP system，meanwhile taking the cell membran e from hepatopancreas，muscle，and E．coli for control group，the result verified the specific binding between W SSV an d gill cell membran e ．And here the binding assay can be employed as a convenient an d rapid way to evaluate the binding nature between W SSV an d the shrimp gill cell membran e．Abstract：Cell membran e extracted from shrimp gill was coated on 96 well plate and bound witIl DIG labeled White spot syndrome virus(wssv)，then detected with Anti—DIG—Fab—HRP system，meanwhile taking the cell membran e from hepatopancreas，muscle，and E．coli for control group，the result verified the specific binding between W SSV an d gill cell membran e ．And here the binding assay can be employed as a convenient an d rapid way to evaluate the binding nature between W SSV an d the shrimp gill cell membran e．
- White spot syndrome virus(wssv)
- , Cell membrane
- , Binding assay

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Verifying the Specific Binding Between W SSV and Shrimp Gill Cell Membrane

1. Yellow Sea Fisheries Research Institute，Chinese Academy ofFishery Sciences,Qingdao 266071，China
2. Ocean UniversityofChina Qingdao 266003，China

Abstract: Abstract：Cell membran e extracted from shrimp gill was coated on 96 well plate and bound witIl DIG labeled White spot syndrome virus(wssv)，then detected with Anti—DIG—Fab—HRP system，meanwhile taking the cell membran e from hepatopancreas，muscle，and E．coli for control group，the result verified the specific binding between W SSV an d gill cell membran e ．And here the binding assay can be employed as a convenient an d rapid way to evaluate the binding nature between W SSV an d the shrimp gill cell membran e．Abstract：Cell membran e extracted from shrimp gill was coated on 96 well plate and bound witIl DIG labeled White spot syndrome virus(wssv)，then detected with Anti—DIG—Fab—HRP system，meanwhile taking the cell membran e from hepatopancreas，muscle，and E．coli for control group，the result verified the specific binding between W SSV an d gill cell membran e ．And here the binding assay can be employed as a convenient an d rapid way to evaluate the binding nature between W SSV an d the shrimp gill cell membran e．