Cytomegalovirus Infection Induces Variation of Cell Cycle and Cyclin E in M RC-5 cells

YAN Yin; CHEN Xiao; YAN Yuan; fan g; YAN Xiao

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August 2003

YAN Yin, CHEN Xiao, YAN Yuan, fan g and YAN Xiao. Cytomegalovirus Infection Induces Variation of Cell Cycle and Cyclin E in M RC-5 cells[J]. Virologica Sinica, 2003, 18(4): 311-314.

Citation: YAN Yin, CHEN Xiao, YAN Yuan, fan g, YAN Xiao. Cytomegalovirus Infection Induces Variation of Cell Cycle and Cyclin E in M RC-5 cells .VIROLOGICA SINICA, 2003, 18(4) : 311-314.

巨细胞病毒感染致MRC-5细胞周期及Cyclin E表达的变化

1.
1．武汉大学医学院病毒研究所湖北武汉，430071：2．武汉市铁道第一中学，湖北武汉，430033

摘要

摘要：本文研究了巨细胞病毒感染人二倍体细胞MRC一5后诱导产生高水平Cyclin E，Cyclin E／cdk2激酶活性增加和导致细胞周期阻滞。应用流式细胞仪分析表明10PFU／cell的病毒量感染MRC一5细胞72h后，29％细胞位于S期， 69％细胞位于G2／M 期，只有2％细胞位于Gl／G0期。应用双抗夹心ELISA法检测，感染病毒20h后，MRC一5细胞中Cyclin E含量比对照细胞高出8倍。感染细胞中CyclinE／cdk2激酶活性基本上与CyclinE含量相关联。
- 巨细胞病毒感染
- , 细胞周期

Cytomegalovirus Infection Induces Variation of Cell Cycle and Cyclin E in M RC-5 cells

1.
The Institute of Virology of the Medical College of Wuhan University,Wuhan 430071，China

Abstract

Abstract：In this Paper we repoaed that Cytomegalovirus infection induced high levels of Cyclin E， increases of Cyclin E／cdk2 activity，leading to cell cycle arrest．By 72h postinfection(at a multiplicity of infection of 1 0 PFU／per cel1)，29％ of the cells was S phase，69％ of the cells in Gz／M phase，only 2％ of the cells in Go／G1 phase by flow cytometry an alysis．By 20h postinfection，the levels of cyclinE in infected M RC．5 cells were eightfold greater than those in mock-infectcd cells by ELISA an alysis．The cyclin E／cdk2 kinase activities in HCM V-infected M RC一5 cells were relative to the levels of cyclinE essentially．
- Cytomegalovirus Infection
- , Cell Cycle

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cytomegalovirus Infection Induces Variation of Cell Cycle and Cyclin E in M RC-5 cells

1. The Institute of Virology of the Medical College of Wuhan University,Wuhan 430071，China

Abstract: Abstract：In this Paper we repoaed that Cytomegalovirus infection induced high levels of Cyclin E， increases of Cyclin E／cdk2 activity，leading to cell cycle arrest．By 72h postinfection(at a multiplicity of infection of 1 0 PFU／per cel1)，29％ of the cells was S phase，69％ of the cells in Gz／M phase，only 2％ of the cells in Go／G1 phase by flow cytometry an alysis．By 20h postinfection，the levels of cyclinE in infected M RC．5 cells were eightfold greater than those in mock-infectcd cells by ELISA an alysis．The cyclin E／cdk2 kinase activities in HCM V-infected M RC一5 cells were relative to the levels of cyclinE essentially．