为鉴定我国森林脑炎病毒亚型，了解基因组结构与生物功能的关系， 同时为森林脑炎病毒新型疫苗研制打 下基础，对森林脑炎病毒森张株编码区序列进行测定。根据已发表的S in-HO、Oshima5．10株序列设计9对重 叠引物，通过RT．PCR扩增不同的cDNA片段，分别克隆至pGEM~-T载体，转化DH5 Q菌，挑阳性克隆PCR、 酶切鉴定后测序。结果表明森张株编码区全长10 245nt，编码3 414个氨基酸。我国森林脑炎病毒森张株与 Oshima5．10、Sofjin．HO、SoOin同源性最近，属于远东亚型。

Abstract：To assign the ck．borne encephalitis virus(TBEV)Senzhang strain isolated in China to one of three TBEV subtypes and understand the relation between the polyprotein structure an d biological function an d aid in the development of new TBEV vaccines．the nucleotide sequence of the polyprotein of Senzhan g strain was determined．Nine pairs of primers were designed according to the nucleotide sequence of Sofjin．HO。Oshima 5·10 strain．By RT·PCR，cDNA fragments of Senzhang strain were acquired from infected BHK．21 cells．Then the cDNA fragments was cloned into the vector pGEM ％T and then tran sforrned to competent DH5 Q cells．Positive clones were screened an d identified by EcoR I cleavage an d PCR．Th e sequences of inserted fragment were determined bv 3 1o0 Genetic Analyzer． Sequence analysis showed that Senzhang strain contains a sigle open reading frame(ORF)of 10245 nucleotides(nt)which encodes a polyprotein of 34 1 4 amino acids(aa)．Senzhang strain is more similar to Oshima5·10，So1]in·HO，So1]in，and assigned to the Far Eastern Subtype．