ZHAO Guang, ZHANG Jia and HU Yuan. Original Study of Korean Isolate of HaSNPV[J]. Virologica Sinica, 2003, 18(4): 348-351.
Citation: ZHAO Guang, ZHANG Jia, HU Yuan. Original Study of Korean Isolate of HaSNPV .VIROLOGICA SINICA, 2003, 18(4) : 348-351.

棉铃虫核型多角体病毒朝鲜分离株的初步研究

  • 摘要:为了充分利用棉铃虫核型多角体病毒(Helicoverpa armigera,HaSNPV)资源,为开展害虫生物防治提供依 据,对首次在朝鲜分离到的棉铃虫核型多角体病毒进行了研究。本文从形态结构,结构多肽。核酸限制性内切酶 图谱等方面进行了研究。多角体直径0.36—1.3pm,平均1.02pm,病毒粒子大小为326 nm×69nm。经SDS—PAGE 分析,棉铃虫核型多角体朝鲜株多角体蛋白为一条带。多角体蛋白分子量为28.7kDa。棉铃虫核型多角体朝鲜株 基因组经BamH I,EcoR I,HindlII和Psi I消化后,得到的内切酶图谱表现,与已报道的几个分离株比较类似。 分子大小均为130.18kb。

Original Study of Korean Isolate of HaSNPV

  • Abstract:Korean isolate was studied on the shape and structure,structural polypeptide,restriction pattern.Diameter of polyhedron is 0.36~1.3Um, size of virion is 326 am × 69nm.Assayed by SDS—PAGE,polyhedron of Korean isolates contained a single polypeptide whose molecular is 28.7kDa. genome is 1 30.1 8kb.Genome digested by BamH I,EcoR I,Hind III and Pst I,map attained is very similar to the other isolates reported.

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    Original Study of Korean Isolate of HaSNPV

    • 1. J.Insect Virology and Structure Virology Lab,College ofLife Science,Wuhan University,Wuhan 430071,China
    • 2. Pyongyang Agriculture iversity,Pyongyang 5643,Democratic Peoples Republic of Korean

    Abstract: Abstract:Korean isolate was studied on the shape and structure,structural polypeptide,restriction pattern.Diameter of polyhedron is 0.36~1.3Um, size of virion is 326 am × 69nm.Assayed by SDS—PAGE,polyhedron of Korean isolates contained a single polypeptide whose molecular is 28.7kDa. genome is 1 30.1 8kb.Genome digested by BamH I,EcoR I,Hind III and Pst I,map attained is very similar to the other isolates reported.

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