Isolation and Identification of a Lethal Viral Pathogen From Com mon Cotton．eared M armosets

SHI Jian—dang; LI Xiao—mian; LIU Feng—yong; JIN Meng-jue; ZHANG Guo-ji; LIU Min; LI Mei

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August 2003

SHI Jian—dang, LI Xiao—mian, LIU Feng—yong, JIN Meng-jue, ZHANG Guo-ji, LIU Min and LI Mei. Isolation and Identification of a Lethal Viral Pathogen From Com mon Cotton．eared M armosets[J]. Virologica Sinica, 2003, 18(4): 357-361.

Citation: SHI Jian—dang, LI Xiao—mian, LIU Feng—yong, JIN Meng-jue, ZHANG Guo-ji, LIU Min, LI Mei. Isolation and Identification of a Lethal Viral Pathogen From Com mon Cotton．eared M armosets .VIROLOGICA SINICA, 2003, 18(4) : 357-361.

一株引起普通棉耳狨猴死亡病毒的分离与鉴定

1.
1．天津医科大学微生物教研室，天津，300070：2．天津医科大学动物中心，天津，300070

摘要

摘要：1999年6月，天津医科大学动物中心饲养的珍贵灵长类实验动物—— 普通棉耳狨猴群体中暴发急性呼吸道传染病，病死率高达33％。在排除细菌感染的基础上，通过死亡狨猴肺组织匀浆接种鸡胚和MDCK 细胞的分离培养，分离出一株具有高血凝效价的病毒株。经双份血清试验及动物接种试验，确认该病毒是本次疾病流行的病原体。又进一步通过与常见呼吸道病毒标准毒株及血清进行交叉血凝抑制试验、电镜观察、RT—PCR 技术并结合生物信息学方法对该毒株进行鉴定，确认本次疾病流行的病原体是副流感1型病毒中的仙台病毒。
- 普通棉耳狨猴
- , 血凝抑制试验
- , RT．PCR
- , BLAST
- , 仙台病毒

Isolation and Identification of a Lethal Viral Pathogen From Com mon Cotton．eared M armosets

1.
Departmrnt of Microbiology,7ianjin Medical University,7ianjin 300070,China

Abstract

Abstract：As the close consan guinity of human race，the common cotton—eared marmoset(Callithrbc Jacchus)has been used in a wide range of research as a primate mode1．Acute respiratory infection(ARI) broke out in a colony reared in the experimental animal center of Tianjin Medical University in 1 999．A virus strain with high hemagglutinin titer was isolated out from embryonated eggs an d M DCK cells after inoculating with the lung tissues suspension of the dead marmosets． The data of the hemagglutination inhibition (HI)test with paried serum samples and the animal inoculation test confirmed that the isolated virus strain is the pathogen of the ARI．Th e isolated virus strain was then identified by the cross HI test。electron microscope observation。RT—PCR an d BLAST analyses．The virus was verified to be Sendai virus，which belongs to Parainfluenza virus type 1．virus was verified to be Sendai virus，which belongs to Parainfluenza virus type 1．
- Common cotton—eared marmoset
- , Hemagglutination inhibition test
- , RT—PCR
- , BLAST
- , Sendai virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of a Lethal Viral Pathogen From Com mon Cotton．eared M armosets

1. Departmrnt of Microbiology,7ianjin Medical University,7ianjin 300070,China

Abstract: Abstract：As the close consan guinity of human race，the common cotton—eared marmoset(Callithrbc Jacchus)has been used in a wide range of research as a primate mode1．Acute respiratory infection(ARI) broke out in a colony reared in the experimental animal center of Tianjin Medical University in 1 999．A virus strain with high hemagglutinin titer was isolated out from embryonated eggs an d M DCK cells after inoculating with the lung tissues suspension of the dead marmosets． The data of the hemagglutination inhibition (HI)test with paried serum samples and the animal inoculation test confirmed that the isolated virus strain is the pathogen of the ARI．Th e isolated virus strain was then identified by the cross HI test。electron microscope observation。RT—PCR an d BLAST analyses．The virus was verified to be Sendai virus，which belongs to Parainfluenza virus type 1．virus was verified to be Sendai virus，which belongs to Parainfluenza virus type 1．