The Cloning And Sequence Analysis of The pl Gene of Potato virus Y

wU Xing—quan; CHEN Shi—hua; wU Zu-jian; LIN Qi—ying; XIE Lian—hui*

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August 2003

wU Xing—quan, CHEN Shi—hua, wU Zu-jian, LIN Qi—ying and XIE Lian—hui*. The Cloning And Sequence Analysis of The pl Gene of Potato virus Y[J]. Virologica Sinica, 2003, 18(4): 376-380.

Citation: wU Xing—quan, CHEN Shi—hua, wU Zu-jian, LIN Qi—ying, XIE Lian—hui*. The Cloning And Sequence Analysis of The pl Gene of Potato virus Y .VIROLOGICA SINICA, 2003, 18(4) : 376-380.

马铃薯Y病毒pl基因的克隆与序列分析

1.
福建农林大学植物病毒研究所，福建福州，35~ 2

摘要

摘要：利用依据马铃薯Y病毒(PVY)pl基因序列设计合成的一对引物YPl，YP2，以带毒烟草总RNA为模板，通过RT-PCR方法扩增得到了0．83kb的目的条带，测序结果表明为PVYpl基因。通过对PVY P1蛋白氨基酸序列分析发现PvY不同分离物间Pl蛋白氨基酸序列存在明显差异，氨基酸序列同源性在64％～94％间。依据Pl蛋白氨基酸序列建立了PVY系统关系树并对PVY进行了类型分析。
- 马铃薯Y病毒
- , pl基因
- , 序列分析

The Cloning And Sequence Analysis of The pl Gene of Potato virus Y

1.
Fuiian Agriculture and Forestry University,Fuzhou 350008．China

Abstract

With the specific primers YP1 and YP2 which were designed based on the reported Potato virus Y(PVY)pl gene sequence，the target gene(0．83kb)was amplified by RT—PCR using the total I A extracted from PVY infected tobacco plant as templates．The gene was cloned into plasmid an d tran sformed then E coli．DH5a sequenced．The result of sequence analysis shows it iS the PVY pJ gene． The comparison shows that there are significant differences on the P1 amino acid sequence am ong the known PVY isolates。the identity ran ges from 64％ tO 94％。Based Oil P1 amino acid sequence，the PVY phylogenic tree was established an d the isolates of PVY were clustered into man y bran ches．
- Potato virus y
- , pl gene
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Cloning And Sequence Analysis of The pl Gene of Potato virus Y

1. Fuiian Agriculture and Forestry University,Fuzhou 350008．China

Abstract: With the specific primers YP1 and YP2 which were designed based on the reported Potato virus Y(PVY)pl gene sequence，the target gene(0．83kb)was amplified by RT—PCR using the total I A extracted from PVY infected tobacco plant as templates．The gene was cloned into plasmid an d tran sformed then E coli．DH5a sequenced．The result of sequence analysis shows it iS the PVY pJ gene． The comparison shows that there are significant differences on the P1 amino acid sequence am ong the known PVY isolates。the identity ran ges from 64％ tO 94％。Based Oil P1 amino acid sequence，the PVY phylogenic tree was established an d the isolates of PVY were clustered into man y bran ches．