Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System

HAO Yong—hua; KUANG Zhi—zhou; XU Yang

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October 2003

HAO Yong—hua, KUANG Zhi—zhou and XU Yang. Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System[J]. Virologica Sinica, 2003, 18(5): 411-414.

Citation: HAO Yong—hua, KUANG Zhi—zhou, XU Yang. Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System .VIROLOGICA SINICA, 2003, 18(5) : 411-414.

轮状病毒vp8基因在原核系统中的初步表达

1.
江西省中德联合研究院，教育部食品重点实验室，江西南昌，330047

摘要

通过RT-PCR反应获得轮状病毒Wa株vp8基因的cDNA片段，将其克隆入pGEX一5X一1表达载体中，构建重组质粒pGEX—VP8，转化大肠杆菌JM109，筛选阳性克隆子并对插入片段vp8进行序列测定，诱导后通过 SDS—PAGE检测重组蛋白，并观察表达量随时间变化的特征。结果显示，测序结果与vp8序列一致，VP8蛋白的表达量在诱导后6-8h达到高峰。
- 轮状病毒Wa,VP8,基因表达

Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System

1.
Joint Research Institute of China—Germany in Jiangxi province，Key Laboratory of Food stuf of Chinese Education Ministry,Nachang 330047，China

Abstract

A cDNA fragment of the vp8 gene from Rotavirus strain Wa was obtained by RT-PCR and cloned into the expressing vector pGEX-5x-1 to construct recombinant plasmid pGEX-VP8. Transformation of pGEX-VP8 to E.coli JM109 and positive clonse was select, The fragment vp8 was sequenced. With IPTG inducing, the recombimant productivity was detected by SDS-PAGE. The results demonstrated that the sequence of vp8 was correct and the productivity of VP8 recombinant protein was peaked after IPTG induction in 6-8h．
- Rotavirus Wa strain

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System

1. Joint Research Institute of China—Germany in Jiangxi province，Key Laboratory of Food stuf of Chinese Education Ministry,Nachang 330047，China

Abstract: A cDNA fragment of the vp8 gene from Rotavirus strain Wa was obtained by RT-PCR and cloned into the expressing vector pGEX-5x-1 to construct recombinant plasmid pGEX-VP8. Transformation of pGEX-VP8 to E.coli JM109 and positive clonse was select, The fragment vp8 was sequenced. With IPTG inducing, the recombimant productivity was detected by SDS-PAGE. The results demonstrated that the sequence of vp8 was correct and the productivity of VP8 recombinant protein was peaked after IPTG induction in 6-8h．