Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

WANG Ping—zhong; BAI Xue—Fan料; PAN Lei; ZHANG Ying; HUANG Chang—xing

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October 2003

WANG Ping—zhong, BAI Xue—Fan料, PAN Lei, ZHANG Ying and HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome[J]. Virologica Sinica, 2003, 18(5): 415-419.

Citation: WANG Ping—zhong, BAI Xue—Fan料, PAN Lei, ZHANG Ying, HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome .VIROLOGICA SINICA, 2003, 18(5) : 415-419.

含HTNV S基因重组痘苗病毒在HFRS患者B淋巴母细胞系中的表达

1.
第四军医大学唐都医院全军感染病诊疗中心，陕西西安，710038

摘要

摘要：采用Ficoll密度梯度离心法(淋巴细胞分离液)分离肾综合征出血热(HFRS)患者外周血单个核细胞(PBMC)，并用EB病毒(EBV)感染B淋巴细胞，建立永生化的B淋巴母细胞系(B—LCL)。然后，用含汉滩病毒(Hantaan virus，HTNV)S基因的重组痘苗病毒感染B—LCL，应用间接免疫荧光检测核衣壳蛋白的表达。结果表明，B淋巴细胞经EBV感染4周左右，可形成永生化B—LCL。成功转化后的B—LCL，体积增大，且增殖的淋巴细胞积聚成团。汉滩病毒s基因在B—LCL中能有效表达核衣壳蛋白。含s基因的重组痘苗病毒感染的B—LCL可用作HTNV 核衣壳蛋白特异性C几活性研究的靶细胞
- 汉滩病毒：重组痘苗病毒
- , 肾综合征出血热
- , EB病毒
- , B淋巴母细胞系

Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

1.
The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA，Tangdu Hospital,Fourth Military Medical University,Xi'an710038．China

Abstract

Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS）and inf~ted by EB virus(EBV)，in Order to establish immortalized B lymphoblastoid cell line(B—LCL)． B—LCLs were then infected with the recombinant vaccinia viruse harboring Hantaan virus fHTNV)S gene．Expression of nucleocapsid protein was detected using indirect immunofluorescent assay(IFA)． The results showed th at imm ortalized B—LCLs were set up．after B lymphocytes were infected by EBV an d cultured for 4 weeks．B lym phocytes tran sform ed by EBV becam e enlar ged an d further gath ered together．The HTNV nucleocapsid protein can be efectively expressed in B—LCLs仃ansinfected with recom—binant vaccinia viruse harboring HTNV-S gene．
- Hantaan virus(HTNv)
- , Recombinant vaccinia virus(rVV)：Hemorhagic Fever with Renal Syndrome(HFRS)
- , EB virus(EBV)
- , B lymphoblastoid cell lines(B—LCL)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

1. The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA，Tangdu Hospital,Fourth Military Medical University,Xi'an710038．China

Abstract: Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS）and inf~ted by EB virus(EBV)，in Order to establish immortalized B lymphoblastoid cell line(B—LCL)． B—LCLs were then infected with the recombinant vaccinia viruse harboring Hantaan virus fHTNV)S gene．Expression of nucleocapsid protein was detected using indirect immunofluorescent assay(IFA)． The results showed th at imm ortalized B—LCLs were set up．after B lymphocytes were infected by EBV an d cultured for 4 weeks．B lym phocytes tran sform ed by EBV becam e enlar ged an d further gath ered together．The HTNV nucleocapsid protein can be efectively expressed in B—LCLs仃ansinfected with recom—binant vaccinia viruse harboring HTNV-S gene．