Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

WANG Ping—zhong; BAI Xue—Fan; PAN Lei; ZHANG Ying; HUANG Chang—xing

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October 2003

WANG Ping—zhong, BAI Xue—Fan, PAN Lei, ZHANG Ying and HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome[J]. Virologica Sinica, 2003, 18(5): 420-422.

Citation: WANG Ping—zhong, BAI Xue—Fan, PAN Lei, ZHANG Ying, HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome .VIROLOGICA SINICA, 2003, 18(5) : 420-422.

中国株HIV-1核心蛋白真核表达载体的构建与表达

1.
第四军医大学唐都医院全军感染病诊疗中心，陕西西安，710038

摘要

摘要：运用限制性内切酶Xba I、Sal I对pKSGAG进行双酶切，获得HIV-1 gag基因，并与真核表达载体pCI-neo 连接，构建含有中国流行株HIV-1核心蛋白真核表达载体pCI．neoGAG。经Xba I／Sal I双酶切及测序鉴定证实，成功地构建了HIV-1核心蛋白真核表达载体pCI．neoGAG。通过脂质体将pCI—neoGAG转染入p815细胞，G418 筛选4周后，使用间接免疫荧光方法检测表达产物。结果表明所构建的HIV-1核心蛋白真核表达载体能在p815 细胞中高效表达，为下一步进行HIV-1 DNA疫苗研究奠定了基础。
- HIV-1：核心蛋白
- , 真核表达载体

Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

1.
The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA，Tangdu Hospital,Fourth Military Medical University,Xi’an 710038．China

Abstract

Abstract：In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG)，gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector pCI．neo．The construction of pCI．neoGAG was confirmed by restriction endonuclease digestion(Xba I ISal I)analysis and DNA sequencing．Th en the pCI-neoGAG was transfected into p8 1 5 cells by lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection for 4 weeks．The results showed that the core protein was expressed in p8 1 5 cells successfully．which will be used for the further test of HIV．1 DNA vaccine．
- Human immunodeficieacy virus I(HIV-1)
- , Core protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

1. The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA，Tangdu Hospital,Fourth Military Medical University,Xi’an 710038．China

Abstract: Abstract：In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG)，gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector pCI．neo．The construction of pCI．neoGAG was confirmed by restriction endonuclease digestion(Xba I ISal I)analysis and DNA sequencing．Th en the pCI-neoGAG was transfected into p8 1 5 cells by lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection for 4 weeks．The results showed that the core protein was expressed in p8 1 5 cells successfully．which will be used for the further test of HIV．1 DNA vaccine．