摘要：利用逆转录一聚合酶链反应(RT-PCR)技术，扩增了人B组轮状病毒WH．2vp7基因，连接到克隆载体pUCm—T， 并对其基因序列进行了分析。WH一2与ADRV的同源性达98％，与印度加尔各达分离株CAL．1达92％，而与动 物B组轮状病毒同源性差别较大，如与IDIR(鼠)同源性仅为58％，与WD653(牛)B组轮状病毒同源性为63％。 与ATI(牛)同源性为61％。对vp7基因的一-~级结构分析发现其mRNA折叠形成多达18个发卡环状结构。vP7 蛋白是249个氨基酸组成，分子量为28．4kDa，含有3个潜在的N连接的糖基化位点和多个磷酰化位点，从氨基 酸序列的同源性来看，WH一2与ADRV的同源性达99％，与CAL一1达95％，而ID 仅为51％，说明了WH．2和 ADRV的起源相同。此研究对了解B组轮状病毒基因的进化和变异规律具有重要意义，也将为B组轮状病毒预防 性疫苗的研制提供科学的依据。

Abstract：The VP7 gene(vp7)ofHuman group B rotavirus(GBRV)strain WH一2 was amplified and cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR)．Positive vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee，the nucleotide sequence of WH一2 vp7 gene showed high identities(more than 9O％)to that of other human GBRV,with low identities(1ess than 65％)to that of animal GBRV．The secondary structure of WH-2 vp7 mRNA was constructed by software RNAstructure 3．7 an d RnaViz 2．0，18 stem—loop structures were found on it． Th e polypeptide encoded by W H一2 vp7 gene contains 249 amino acids with a calculated molecular weight of 28．4 kDa．The protein contains three potential N—linked glycosylation sites an d a hydrophobic signal—liked sequence at its amino termina1．Comparied with other GBRV,WH一2 vp7 demonstrated 99％ an d 95％ of am ino acids similarity with ADRV and CAL．1 respectively,only 5 1％ witIl IDⅡ ．Th ese findings suggested that W H一2 and ADRV might originate from a common an cestral virus，district from an imal W H一2 vp7 reported SO far．