DAI Chunming, ZHANG Xiaoyan, SHAO Yiming and SHEN Rongxian. Study on Immunity of Recombinant Vaccinia Viruses Containing env Gene of EIAV[J]. Virologica Sinica, 2003, 18(5): 473-477.
Citation: DAI Chunming, ZHANG Xiaoyan, SHAO Yiming, SHEN Rongxian. Study on Immunity of Recombinant Vaccinia Viruses Containing env Gene of EIAV .VIROLOGICA SINICA, 2003, 18(5) : 473-477.

EIAV env基因在痘苗病毒中的表达及其免疫效果的观察

  • 摘要:将马传贫驴白细胞弱毒疫苗及其亲本株env基因克隆到痘苗病毒表达载体pSC65的pE/L启动子下游,通 过同源重组插入到痘苗病毒天坛株基因组TK 区,经蓝白斑筛选获得重组痘苗病毒r、,v.DLVenv和rw.LNenv, Western blot检测目的蛋白的表达,结果表明重组痘苗病毒能够有效表达完整的EIAV Env蛋白,其肌肉接种免疫 小鼠后,表达的目的蛋白具有良好的免疫原性,能够诱导机体产生有效的体液和细胞免疫应答,其中以细胞免疫 效果更为显著,CTL特异性裂解最高可达28%。本研究为EIAV基因工程疫苗的开发研制奠定了基础。

Study on Immunity of Recombinant Vaccinia Viruses Containing env Gene of EIAV

  • In order to develop new vaccine candidate of Equine infectious anema virus(EIAV),env gene of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the downstream of pE/L promoter of pSC65 vector,separately.The resulted recombinant vaccinia viruses were screened out by homologous recombination in TK region and the positive clone was confirmed with blue plaque assay.Protein expression was examined by W estern blot.It was shown that the recombinant vaccinia viruses successfully expressed the complete EIAV Env proteins an d induced significan t cellular an d humoral immune responses in the Balb/c mi ce by intramuscular inoculation. CTL specific lysis is 28% at most.Th e study will provide importan t elements for development of new EIAV genetic engineering vaccine.

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    Study on Immunity of Recombinant Vaccinia Viruses Containing env Gene of EIAV

    • 1. Division ofResearch on Virology and Immunology,National Centerfor AIDS/STD Control and Prevention.CDC, Peking 100050,China

    Abstract: In order to develop new vaccine candidate of Equine infectious anema virus(EIAV),env gene of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the downstream of pE/L promoter of pSC65 vector,separately.The resulted recombinant vaccinia viruses were screened out by homologous recombination in TK region and the positive clone was confirmed with blue plaque assay.Protein expression was examined by W estern blot.It was shown that the recombinant vaccinia viruses successfully expressed the complete EIAV Env proteins an d induced significan t cellular an d humoral immune responses in the Balb/c mi ce by intramuscular inoculation. CTL specific lysis is 28% at most.Th e study will provide importan t elements for development of new EIAV genetic engineering vaccine.

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