摘要：OaEPV经2×SDS包涵体碱性裂解缓冲液裂解，离心，分别用上清液及沉淀与绿僵菌孢子混合感染黄胫小 车蝗，上清液的增效活性比沉淀大2．5倍。用三种不同方法(2×SDS包涵体碱性裂解液法，0．02 mol／LNaOH裂 解法，8 mol／L尿素裂解法)裂解OaEPV所得蛋白液与绿僵菌混用，生测结果表明，包涵体裂解液法制备所得蛋 白增效活性最高，NaOH裂解法次之，尿素裂解法制备的蛋白几乎没有增效活性。将OaEPV 用包涵体碱性裂解 液裂解后，用葡聚糖G一200凝胶柱层析进行分离，出现两个洗脱峰，这两个峰的洗脱液浓缩后进行生物测定，初 步表明增效作用主要为第二个峰的蛋白片段。经SDS一聚丙烯酰胺凝胶电泳，第二个峰的蛋白片段的分子量为40 kDa左右。

Abstract：Oedaleus asiaticus entomopoxvirus(DaEPV) contains a factor which enhances the infection of Metarhizium flaroviride in the grasshopper Oedaleus infernalis．When the spheroids of OaEPV were dissolved with 2×SDS spheroid alkaline solution and centrifuged，the enhancing activity of the supernatant was 2．5 times higher than in the pellet．The enhancing activity of spheroid proteins depended on the alkali used to dissolve the spheroids．The greatest activity was detected with spheroid alkaline solution，followed with 0．02 mol／L NaOH，and none with 8 mol／L urea．Spheroid proteins were fractionated on a sephadex G一200 column．Two fractions were obtained an d the second fraction had the enhancing activity．SDS—PAGE indicated the molecular weight of the active fraction was40 kDa．