WANG Xiao—fan, WANG Zhi—yu, XUE Yong—lei and YAO Ping. Expression Analysis of Envelope Glycoprotein E2 Protein of Rubella virus JR23 Strain[J]. Virologica Sinica, 2003, 18(5): 500-502.
Citation: WANG Xiao—fan, WANG Zhi—yu, XUE Yong—lei, YAO Ping. Expression Analysis of Envelope Glycoprotein E2 Protein of Rubella virus JR23 Strain .VIROLOGICA SINICA, 2003, 18(5) : 500-502.

风疹病毒JR23株包膜糖蛋白E2重组体的构建表达与抗原性分析

  • 风疹病毒(Rubella virus,RV)是人类最重要、最 常见的致畸病毒之一,但其致畸机理至今尚未完全 明了[11 o RV的包膜上镶嵌着两个糖蛋白E2和El, E2/E1以异二聚体的形式在病毒体表面形成刺突结 构I引。E2的糖基化程度很高,存在O一联及N一联糖 基化位点,糖类在成熟的E2蛋白中所占比重大于 三分之一,E2蛋白的糖基化程度影响蛋白功能,糖 基化位点的改变可能与RV减毒有关。

Expression Analysis of Envelope Glycoprotein E2 Protein of Rubella virus JR23 Strain

  • To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23 strain,to express E2 protein in BHl(2 l cells,and to analyze the antigenic sites of E2 protein of RV JR23 strain.E2 gene of RV JR23 strain was amplified bv I PCR.The PCR product was cloned into the expression vector pBluescript II S and transformed into E.coli JM 109.an d the recombinant plasmid was transfected into BHK21 cells with recombinant vaccinia viru s VTF一7.The expression was detected by immunohistochemistry an d indirect immunofluorescence.The recombinan t plasmid contained glycoprotein E2 gene.and expressed well in BHK2 l cells.The expressed E2 protein mainly accumulated in plasma near the nuclei.Th ere are immunological epitopes of E2 protein on RV JR23 virions.The results showed that RV JR23 E2 transient expression system was successfully constructed. This study ofers the basis for investigation of the relationship between the structure an d fu nction of RV proteins,and interaction of viru s an d host cells.

  • 加载中
  • 加载中

Article Metrics

Article views(4044) PDF downloads(833) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Expression Analysis of Envelope Glycoprotein E2 Protein of Rubella virus JR23 Strain

    • 1. Department of Virology,School of Public Health,Shandong University,Jinan 250012,China

    Abstract: To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23 strain,to express E2 protein in BHl(2 l cells,and to analyze the antigenic sites of E2 protein of RV JR23 strain.E2 gene of RV JR23 strain was amplified bv I PCR.The PCR product was cloned into the expression vector pBluescript II S and transformed into E.coli JM 109.an d the recombinant plasmid was transfected into BHK21 cells with recombinant vaccinia viru s VTF一7.The expression was detected by immunohistochemistry an d indirect immunofluorescence.The recombinan t plasmid contained glycoprotein E2 gene.and expressed well in BHK2 l cells.The expressed E2 protein mainly accumulated in plasma near the nuclei.Th ere are immunological epitopes of E2 protein on RV JR23 virions.The results showed that RV JR23 E2 transient expression system was successfully constructed. This study ofers the basis for investigation of the relationship between the structure an d fu nction of RV proteins,and interaction of viru s an d host cells.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return