Cloning and Expression of the Nonstructural Protein(NS 1)of the H9N2 Chicken Influenza Virus

LIU Jin—hua; WU Qing—min; SHI Wei—min; GUO Yu—pu

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October 2003

LIU Jin—hua, WU Qing—min, SHI Wei—min and GUO Yu—pu. Cloning and Expression of the Nonstructural Protein(NS 1)of the H9N2 Chicken Influenza Virus[J]. Virologica Sinica, 2003, 18(5): 503-505.

Citation: LIU Jin—hua, WU Qing—min, SHI Wei—min, GUO Yu—pu. Cloning and Expression of the Nonstructural Protein(NS 1)of the H9N2 Chicken Influenza Virus .VIROLOGICA SINICA, 2003, 18(5) : 503-505.

H9N2亚型禽流感病毒非结构蛋I~t(NS 1)基因的克隆与表达

1.
中国农业大学农业部预防兽医学苇点开放实验室，北京，100094

摘要

由于H9N2亚型禽流感对我国的养鸡业已造成了很大的损失，因而在我国许多养鸡场不得不使用 H9N2亚型禽流感灭活苗⋯，但由于灭活苗的使用，而增加了H9N2亚型禽流感的监测难度。因此，建立一种能区别自然感染鸡和疫苗免疫鸡的鉴别诊断方法已被提到日程上来。
- 鸡流感病毒
- , 非结构蛋白：基因表达

Cloning and Expression of the Nonstructural Protein(NS 1)of the H9N2 Chicken Influenza Virus

1.
LIU Jin—hua，WU Qing—min，SHI Wei—min，GUO Yu—pu

Abstract

Abstract：The nostructural protein(NS 1)encoded by gene 8 of the Influenza virus is present in cells infected with Influenza virus．In this study,NS 1 protein gene of the Chicken influenza virus A／chicken／Beijing／2／97(H9N2)strain was amplified by PCR．The fragment contains EcoR I and Xho I restriction enzyme sites at the ends．The amplified product was cloned into the expression vector pET-30(c)．Recombinant plasmid pET／NS 1 was transformed into E．coli BL2 1(DE3)competent cells an d induced with 0．4 mmol／L IPTG the target protein was produced．the molecular weight of the expressed protein was 30 kDa as expected．Western—blot test indicated that the expressed protein Can react with the NS 1 monoclonal antibody of the influenza virus．This study laid an importan t foundation for H9N2 subtype avian influenza surveillance in China．
- Chicken influenza virus
- , NS 1：Gene expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Expression of the Nonstructural Protein(NS 1)of the H9N2 Chicken Influenza Virus

1. LIU Jin—hua，WU Qing—min，SHI Wei—min，GUO Yu—pu

Abstract: Abstract：The nostructural protein(NS 1)encoded by gene 8 of the Influenza virus is present in cells infected with Influenza virus．In this study,NS 1 protein gene of the Chicken influenza virus A／chicken／Beijing／2／97(H9N2)strain was amplified by PCR．The fragment contains EcoR I and Xho I restriction enzyme sites at the ends．The amplified product was cloned into the expression vector pET-30(c)．Recombinant plasmid pET／NS 1 was transformed into E．coli BL2 1(DE3)competent cells an d induced with 0．4 mmol／L IPTG the target protein was produced．the molecular weight of the expressed protein was 30 kDa as expected．Western—blot test indicated that the expressed protein Can react with the NS 1 monoclonal antibody of the influenza virus．This study laid an importan t foundation for H9N2 subtype avian influenza surveillance in China．