LIU Zhao, YANG Zhan, XIAO Hong, WEN Lil and WANG Zhen. Antiviral Activity of Algefacient and Anti-inflammatory Against Influenza Virus in vitro and in vivo[J]. Virologica Sinica, 2003, 18(6): 534-537.
Citation: LIU Zhao, YANG Zhan, XIAO Hong, WEN Lil, WANG Zhen. Antiviral Activity of Algefacient and Anti-inflammatory Against Influenza Virus in vitro and in vivo .VIROLOGICA SINICA, 2003, 18(6) : 534-537.

清热消炎复方制剂抗流感病毒作用的研究

  • 为评价清热消炎复方制剂(简称AI)的抗流感病毒活性,我们以病毒唑为对照,通过在体外观察病毒致细胞 病变效应(CPE)、MTT细胞染色检查病毒抑制率和检测病毒血凝滴度;在体内观察其对染毒小鼠的死亡保护作 用,对小鼠流感病毒性肺炎的抑制作用,以及对小鼠肺内病毒增殖的影响,从而判定其抗流感病毒作用。结果发 现AI在160ug/mL时能完全抑制流感病毒在MDCK细胞内的增殖复制作用。体内实验中0.1 gg,O.5gg,1.2g/kg 3个剂量均能明显降低染毒小鼠的致死率,延长平均存活时间;降低肺炎小鼠的肺指数和血凝滴度(P0.01)。其 作用与病毒唑相当。结论认为清热消炎复方制剂是一种有效的体内、体外抗流感病毒中药复方制剂。 Abstract:To observe the antiviral activity of Algefacient and anti-inflammatory(AI)against influenzavirus,cell culture technique was used in M DCK cells to get virus inhibitory rate an d virus tiler by MTTassay an d hemagglitination test.Influenza virus infection model was established in mice,to observe theprotection efect to mi ce death ,the inhibitory efect to pulmonary index and pulmonary virus titer.Theresults showed that 160ug/mL Algefacient an d anti-inflammatory Can completely inhibit theproliferation of influenza virus in MDCK cel1.0.1 gg, 0.5g/kg.1.2g,/kg dosage med cme orally givenfor 5 days could significan tly decrease the mortality rate,prolong the living time of infec ted mi ce,decrease the pulmonary index and pulmonary virus hemagglitination titer(PO.oi).This efect issimilar to that of virazole at the same dosage leve1.W e conclude that AI is an efective anti-influenz avirus.compound/n vitro and in vivo.

Antiviral Activity of Algefacient and Anti-inflammatory Against Influenza Virus in vitro and in vivo

  • Abstract:To observe the antiviral activity of Algefacient and anti-inflammatory(AI)against influenza virus,cell culture technique was used in M DCK cells to get virus inhibitory rate an d virus tiler by MTT assay an d hemagglitination test.Influenza virus infection model was established in mice,to observe the protection efect to mi ce death ,the inhibitory efect to pulmonary index and pulmonary virus titer.The results showed that 160ug/mL Algefacient an d anti-inflammatory Can completely inhibit the proliferation of influenza virus in MDCK cel1.0.1 gg, 0.5g/kg.1.2g,/kg dosage med cme orally given for 5 days could significan tly decrease the mortality rate,prolong the living time of infec ted mi ce, decrease the pulmonary index and pulmonary virus hemagglitination titer(PO.oi).This efect is similar to that of virazole at the same dosage leve1.W e conclude that AI is an efective anti-influenz a virus.compound/n vitro and in vivo.

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    Antiviral Activity of Algefacient and Anti-inflammatory Against Influenza Virus in vitro and in vivo

    • 1. TheInstituteof~rology,theMedicalSchool,Wuhan University,Wuhan 430071,China
    • 2. GuilinSanjin Pharmaceutical Co.LTD,GuHin 541004.china

    Abstract: Abstract:To observe the antiviral activity of Algefacient and anti-inflammatory(AI)against influenza virus,cell culture technique was used in M DCK cells to get virus inhibitory rate an d virus tiler by MTT assay an d hemagglitination test.Influenza virus infection model was established in mice,to observe the protection efect to mi ce death ,the inhibitory efect to pulmonary index and pulmonary virus titer.The results showed that 160ug/mL Algefacient an d anti-inflammatory Can completely inhibit the proliferation of influenza virus in MDCK cel1.0.1 gg, 0.5g/kg.1.2g,/kg dosage med cme orally given for 5 days could significan tly decrease the mortality rate,prolong the living time of infec ted mi ce, decrease the pulmonary index and pulmonary virus hemagglitination titer(PO.oi).This efect is similar to that of virazole at the same dosage leve1.W e conclude that AI is an efective anti-influenz a virus.compound/n vitro and in vivo.

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