Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell

JIAO Cheng．Song; WANG Sheng．Qi; ZHANG He．Qiu; LING Shi．Gan

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December 2003

JIAO Cheng．Song, WANG Sheng．Qi, ZHANG He．Qiu and LING Shi．Gan. Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell[J]. Virologica Sinica, 2003, 18(6): 538-540.

Citation: JIAO Cheng．Song, WANG Sheng．Qi, ZHANG He．Qiu, LING Shi．Gan. Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell .VIROLOGICA SINICA, 2003, 18(6) : 538-540.

HCV全长基因真核表达载体的构建及其细胞内表达

1.
1、广州军区武汉总医院感染内科，湖北武汉，430070
2.
军事医学科学院基础医学研究所，北京，100850

摘要

本实验成功构建了HCV全长基因的真核表达载体pCI·HCVFL，转染HepG2细胞后经免疫荧光和免疫组化法分别检测到结构基因区(c)和非结构基因区(NS3)病毒蛋白的表达，该栽体可用于建立HCV转基因细胞模型以及进一步开展有关HCV复制与表达的深入研究。
- 丙型肝炎病毒：全长基因
- , 蛋白表达

Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell

1.
Department ofInfectious Diseases,Wuhan General Hospital,Guangzhou Military Command,Wuhan 430070,China
2.
AcademyofMilitaryMedicalSciences，Beifing 100850,China

Abstract

An eukaryotic vector containing the full—length Hepatitis C virus(HCV)gene，pCi—HCV，was successfully constructed in this study．After~ansfected into HepG2 cells．it Was revealed to expleSS HCV C and NS3 proteins in the cloned cells by immunofluorescence and immunohistochemistry as Say respectively．It could be used for the experiments of the construction of HCV transgenic cellular model and the further study on the replication of HCV through cDNA．
- Hepatitis C virus
- , Fu11．1ength eDNA：Protein expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell

1. Department ofInfectious Diseases,Wuhan General Hospital,Guangzhou Military Command,Wuhan 430070,China
2. AcademyofMilitaryMedicalSciences，Beifing 100850,China

Abstract: An eukaryotic vector containing the full—length Hepatitis C virus(HCV)gene，pCi—HCV，was successfully constructed in this study．After~ansfected into HepG2 cells．it Was revealed to expleSS HCV C and NS3 proteins in the cloned cells by immunofluorescence and immunohistochemistry as Say respectively．It could be used for the experiments of the construction of HCV transgenic cellular model and the further study on the replication of HCV through cDNA．