摘要：采集急性期病人的咽拭子或漱口液，用Vero、Vero E6、MDCK、Hela、Hep．2等传代细胞，人胚肺二倍 体细胞(髓L)和人胚肺(1iP)细胞分离培养严重急性呼吸系统综合症(SARS)的病原体。结果用Vero、Vero E6、 MDCK和HP细胞从标本中分离到一株病毒。间接免疫荧光试验发现，恢复期病人血清可与所分离的病毒起反应， 在胞膜和胞浆中出现翠绿色荧光；中和试验结果表明，恢复期病人血清能中和病毒对细胞的致细胞病变作用；电镜 下可观察到冠状病毒样颗粒；RT-PCR 法可扩增到冠状病毒特异性基因片段，且其核苷酸序列与国内外发表的 SARS冠状病毒(SARS。Coy)相应的基因序列相符，同源性达到100％。从传染性非典型肺炎病人的漱口液中分 离到SARS冠状病毒，这种病毒与传染性非典型肺炎密切相关。

Abstract：To isolate and identify the etiologic agent of severe acute respiratory syndrome(SARS)， the throat swabs an d gargle of patients with SARS were collected．We inoculated the specimens onto a number of cell lines including Vero，Vero E6，M DCK，Hela，Hep一2，HP an d HEL cells．The isolates were identified by thin—section electron．microscopy，indirect．immunofluorescence，neutralization test, RT—PCR an d nucleotide sequence an alysis．Using cell culture techniques．a coronavirus was isolated from patient with SARS．Four celllines，Vero，Vero E6，HP an d MDCK cells，showed cytopathic efec t． 1]he infected cells showed strong cytoplasmi c an d membran ous staining with a convalescent—phase seruln from the patients with SARS in an indirect—immunofluorescence staining．The cytopathic efect of the viruses clould been neutralized by convalescent serum．Under elec tron—microscopy,a large num be r of coronavirus—like particles were obsewed in the infected cells．W ith specific primers．the specific cDNA fragments were amplified by reverse transcription—polymerase chain reaction(RT—PCR1 from infected cels an d the nucleotide sequence analysis showed that homology of the amplified fragm ents to the SARS—coronavirus(SARS·CoV)previously reported was 100％．A SARS—CoV Was isolated from patients with SARS．T}liS virus was closely associated with SARS．