摘要：用分子克隆技术从兔出血症病毒(RHDV)中国早期流行株NJ85中成功克隆出vp60基因，序列分析表明 基因长度为1740nt，编码579aa。利用GenBank数据库，NJ85与WX84、TP二个RHDV中国毒株vp60基因DNA 序列的同源性分别为92．7％和97．2％，氨基酸序列的同源性分别为96．1％和98．6％，与其他国家l6个毒株的vp60 基因DNA序列的同源性在83．7％~97．0％之间，氨基酸序列的同源性在90．5％~99．0％之间，具有高度的同源性。进一步分析vp6O基因的六个分区，A、B、D、F四个区变异率较低，C、E二个区变异率较高。在遗传进化上，历年来的RHDV毒株在氨基酸水平上分析可分为三个支谱系，在核苷酸水平上趋向四个支谱系，谱系没有呈现地域或时间特征。三个中国毒株分布在二个不同的支谱系中。与RHDV 同为兔病毒属的欧洲野兔综合征病毒(EBHSV)组成了另一个谱系。运用生物信息学方法分析了NJ85 VP60蛋白的分子量、等电点、疏水性和二级结构，根据同源模型预测分析T-级结构。NJ85 VP60的二级结构以B片层为主，三级结构稳定。病毒衣壳表面有32个杯状凹陷， 由9o个二聚体组成，二聚体由VP60单体以A／B5和C／C2两种方式构成，单体在二聚体中的构象有A、B、C三种形式。单体含有S、P两个结构域，两者通过柔性绞链连接，P结构域由VP60的C端部分形成，P分为P1和P2二个亚结构域，P2位于病毒衣壳表面，含有病毒株特异性抗原表位和红细胞结合位点。

Abstract：The capsid protein gene(vp60)of Rabbit hemorrhagic disease virus(RHDV)isolate NJ85 was amplified and sequenced．vp60 gene of NJ85 was in size of 1 740nt an d encoding 579aa． Comparison with other Chinese field isolates W X84 an d TP showed that the homology were 92．7％ an d 97．2％ for nucleofide sequence；96．1％ an d 98．6％ for amino acid sequence respectively．Alignment with other 16 sequences of RHDV isolated from other countries registe in GenBank showed that the homology was 83．7％ ～ 97．0％ for nucleotide and 90．5％~ 99．0％ for amino acid sequence．The results indicated that the sequence of vp60 in diferent RHDV isolates was highly homologous．In the all six regions ofvp60 gene，low degree ofvariation ofvp60 was found in the regions A，B，D，F and relative high degree of variation in the regions C and E．Based on vp60 sequence，phylogenetic an alysis showed that the historic RHDV isolates were divided into 3 branches of the lineage of RHDV according to the am ino acid sequences and 4 branches according to the nucleotide sequences．Th ere was no obvious correlation between geographical region，historic period and homological degree．Three Chinese field isolates lay in 2 diferent branches of the RHDV lineage，while EBHSV formed an other fineage．Th e molecular weight，isoelectric point，hydrophobicity,2-dimensional an d 3-dimensional structure of NJ85 vp60 were de~rmined and predicted on the theory of bioinformatics．The major secondary structure，13 一sheet，contributed to the stability of vp60．Based on the structural model，the architecture of NJ85 capsid was 32 cup—shaped depressions．Th e capsid was composed of 90 A／B5 an d C／C2 dimers formed by the single unit of vp60．Th e conformation of the single unit in dimer existed in thre forms of A，B and C．Th e single unit of Vp60 had a protruding(P)domain connected by a flexible hinge to a shell(S) domain．P domain was subdivided into two subdomains，P1 an d P2．P2 subdomain that located at the surface of the capsid contained the determinants of strain specificity an d erythrocyte binding site．