Isolation and Identification of a M ouse Poxvirus an d Its Infectivit

AN Xue-fang; LIU Feng-song; FANG Ming-gang; ZHU You-; WANG Han-zhong

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December 2003

AN Xue-fang, LIU Feng-song, FANG Ming-gang, ZHU You- and WANG Han-zhong. Isolation and Identification of a M ouse Poxvirus an d Its Infectivit[J]. Virologica Sinica, 2003, 18(6): 563-565.

Citation: AN Xue-fang, LIU Feng-song, FANG Ming-gang, ZHU You-, WANG Han-zhong. Isolation and Identification of a M ouse Poxvirus an d Its Infectivit .VIROLOGICA SINICA, 2003, 18(6) : 563-565.

鼠痘病毒的分离鉴定及感染性研究

1.
1．中国科学院武汉病毒所分子病毒学重点实验室，湖北武汉，430071
2.
湖北郧阳医学院实验动物中心，湖北十堰，442000

摘要

分别从自然感染死亡的小鼠不同病理组织内分离到一种大小为250~300nmX160~190nm 的卵圆型病毒粒子。以腹腔注射的方式将纯化的病毒粒子回复接种到小鼠体内，能引起小鼠死亡，从不同的病理组织内分离到与接种病毒粒子相同的病毒粒子。病理组织的超薄切片电镜观察结果表明在肝、肺、脾、肠等组织的细胞质内均能观察到病毒粒子的存在，表明病毒的复制和装配是在细胞质内完成的，该病毒应归类于痘病毒科。
- 痘病毒
- , 形态学
- , 感染性

Isolation and Identification of a M ouse Poxvirus an d Its Infectivit

1.
The Key Laboratory of Molecular Virology,Wuhan lnsititute of Virology,The Chinese Academy ofSciences,Wuhan 430071。China 2．Experimental Animal Center,Yunyang Medical College in Hubei Province,Shiyan 442000,China

Abstract

Mousepoxvirus has been isolated from diferent pathological tissues in deed mouse that natively infected by virus．In negative stained preparations．the size of the virus particles Was l60～ 19Ohm X250~ 300nm and the virus the particle revealed ovoid·shape．Tl1e healthy Blab／C mouse were infected by injection with purified virus particles，resulting in the death of mouse and producing the same virus particles as inoculated virus．Ttle electron microscopy of an ultrathin section confirmed that viral replication an d assembly of virions occurred in the cytoplasm of diferent pathological tissues infec ted by viruses such as liver,lung．kidney,intestine an d pan creas．These results demonstratedⅡlat the virus should been classified into Poxviridae．
- Poxvirus
- , M orphology
- , Infectivity

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of a M ouse Poxvirus an d Its Infectivit

1. The Key Laboratory of Molecular Virology,Wuhan lnsititute of Virology,The Chinese Academy ofSciences,Wuhan 430071。China 2．Experimental Animal Center,Yunyang Medical College in Hubei Province,Shiyan 442000,China

Abstract: Mousepoxvirus has been isolated from diferent pathological tissues in deed mouse that natively infected by virus．In negative stained preparations．the size of the virus particles Was l60～ 19Ohm X250~ 300nm and the virus the particle revealed ovoid·shape．Tl1e healthy Blab／C mouse were infected by injection with purified virus particles，resulting in the death of mouse and producing the same virus particles as inoculated virus．Ttle electron microscopy of an ultrathin section confirmed that viral replication an d assembly of virions occurred in the cytoplasm of diferent pathological tissues infec ted by viruses such as liver,lung．kidney,intestine an d pan creas．These results demonstratedⅡlat the virus should been classified into Poxviridae．