YU Hong, KONG Xiaohong, XUAN Chenghao, WANG Jinzhong, CHEN Qi—rain and GENG Yun-qi. The Study on the Infection of Rabbits with Bovine Foamy Virus 3026’[J]. Virologica Sinica, 2003, 18(6): 566-570.
Citation:
YU Hong, KONG Xiaohong, XUAN Chenghao, WANG Jinzhong, CHEN Qi—rain, GENG Yun-qi.
The Study on the Infection of Rabbits with Bovine Foamy Virus 3026’ .VIROLOGICA SINICA, 2003, 18(6)
: 566-570.
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摘要
将牛泡沫病毒(BFV3026)感染的细胞经耳缘静脉注射兔子,并以正常细胞注射的兔为对照。1年后处死, 病毒挽救实验及PCR检测显示:兔经一次注射即可被BFV3026感染,病毒广泛分布于感染兔的多种脏器中,通 过共培养可从感染兔血、肝、脾、肺、肾中拯救出相应感染性病毒颗粒,并在脑、骨髓、心、胰、肠系膜中检到 高拷贝BFV原病毒DNA存在。同时,血清学检测表明:感染兔在接受注射一个月后即产生高滴度抗病毒蛋白抗 体,并维持该滴度水平直至实验终止,兔未表现任何可观病变。
Abstract:Rabbits were injected with a single intravenation of Bovine foamy virus 3026(BFV3026)· infected cells in the ear and monitored for sera-conversion serologically up t0 l year.Results of the serological an d virus-rescue assays indicated that all animals were persistently infected with BFV3026 an d showed sustained BFV3O26.spec ific humoral immune response.BFv3026 Was rescued by co-cultivation with fetal bovine lung(FBL)cells from the spleen,kidney,lung,liver and peripheral blood leukocytes of the infected an imals.Virus sequence in poZ was、recovered an d amplified from the tissues where the virus Was rescued from,such as from the heart,brain,bone marrow,mesentery an d the pan creas.In addition.BFv transcripts could be detected in PBL by RT.PCR. S animal model might be usefulfor studyingtheinteractionbe tweenBFv an dBIV
The Study on the Infection of Rabbits with Bovine Foamy Virus 3026’
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Abstract
Abstract:Rabbits were injected with a single intravenation of Bovine foamy virus 3026(BFV3026)· infected cells in the ear and monitored for sera-conversion serologically up t0 l year.Results of the serological an d virus-rescue assays indicated that all animals were persistently infected with BFV3026 an d showed sustained BFV3O26.spec ific humoral immune response.BFv3026 Was rescued by co-cultivation with fetal bovine lung(FBL)cells from the spleen,kidney,lung,liver and peripheral blood leukocytes of the infected an imals.Virus sequence in poZ was、recovered an d amplified from the tissues where the virus Was rescued from,such as from the heart,brain,bone marrow,mesentery an d the pan creas.In addition.BFv transcripts could be detected in PBL by RT.PCR. S animal model might be usefulfor studyingtheinteractionbe tweenBFv an dBIV
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References
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Proportional views
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